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pCDNA3-SREBP-2-GFP (A1134-1135)
Plasmid#204203PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3 and eGFPExpressionMammalianMutationGly to Ala 1134, 1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-2 (STOP)
Plasmid#204204PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3ExpressionMammalianMutationNonsense mutations after Gly1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)
Plasmid#204199PurposeMammalian expression of SREBP-1 (isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTags3xHA, eGFP and T7x2ExpressionMammalianMutationGly to Ala 317,318,341,1171PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-2 (A1134-1135)
Plasmid#204201PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3ExpressionMammalianMutationGly to Ala 1134 to 1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 (A317-318)
Plasmid#204193PurposeMammalian expression of SREBP-1 (isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317, 318PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 (A317-318; A341)
Plasmid#204194PurposeMammalian expression of SREBP-1 (Isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317,318,341PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3- SREBP-1 (A317-318; L340)
Plasmid#204195PurposeMammalian expression of SREBP-1 (Isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317,318; Gly to Leu 340PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3- SREBP-1 (A317-318;A341; A1171)
Plasmid#204196PurposeMammalian expression of SREBP-1 (isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317,318,341,1171PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 (A341; A1171)
Plasmid#204197PurposeMammalian expression of SREBP-1 (isoform 1)DepositorInsertSREBP-1a (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 341, 1171PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)
Plasmid#210767PurposeMammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationDRSR 514-517 to AS; 557 R to A; multiple silent m…PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCINeo-gp78 Mutant G2BR
Plasmid#185355PurposeAssessing cellular requirement for G2BRgp78DepositorInsertgp78 (AMFR Human)
UseTagsGSTExpressionMammalianMutationaa 595-600 KRFLNK to AGAAGGPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 AUP1 61-410
Plasmid#185334PurposeBacterial expression of AUP1 with GST fusion protein for binding assaysDepositorInsertAUP1 (AUP1 Human)
UseTagsGSTExpressionBacterialMutationaa 61-610 onlyPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-gp78 RING
Plasmid#185353PurposeRING domain-containing GST fusion protein for ubiquitination assays.DepositorInsertgp78 cytoplasmic domain (AMFR Human)
UseTagsGSTExpressionBacterialMutationStop codon after aa 393PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3a-UBE2G2
Plasmid#204209PurposeBacterial expression of UBE2G2DepositorInsertUBE2G2 (Ube2g2 Mouse)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-gp78 429-611
Plasmid#185354PurposeGST fusion protein for quantification of gp78 levelsDepositorInsertgp78 (AMFR Human)
UseTagsGSTExpressionBacterialMutationaa 429-611 onlyPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 AUP1 292-410 5E
Plasmid#185332PurposeBacterial expression of AUP1 with GST fusion protein for binding assaysDepositorInsertAUP1 (AUP1 Human)
UseTagsGSTExpressionBacterialMutationaa 292-410 only, with mutations R382E/Q383E/K390E…PromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only
