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pISKP1-TIR1

Plasmid

#165061

Purpose

Integrative expression of SKP1-fusing rice auxin receptor gene OsTIR1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1- T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP105

Plasmid

#165060

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP104

Plasmid

#165059

Purpose

Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID9L104

Plasmid

#165054

Purpose

Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pWDY1228

Plasmid

#138594

Purpose

Expresses NatL2-R156K in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Mutation

changed Arginine 156 to Lysine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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C-13-BE

Plasmid

#135041

Purpose

C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_4

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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C-7-BE

Plasmid

#135035

Purpose

C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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C-6-BE

Plasmid

#135034

Purpose

C-T base editing using LjDA1L2_2 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjDA1L2_2

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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C-11-BE

Plasmid

#135039

Purpose

C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L2_2

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176269

Purpose

Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCasMINI-V4-VPR-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBAMD1-4

Plasmid

#61565

Purpose

Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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MaLionG

Plasmid

#113906

Purpose

Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

Plasmid

#176271

Purpose

Expression of CasMINI-V3.1 in mammalian cells.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI-V3.1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK151

Plasmid

#183127

Purpose

BTK Type 4 reverse promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse CP25 promoter and forward rpoC terminator for dsRNA expression

Use

Synthetic Biology

Available Since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK150

Plasmid

#183126

Purpose

BTK Type 2 promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression

Use

Synthetic Biology

Available Since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MaLionR

Plasmid

#113908

Purpose

Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionR

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBAMD1-6

Plasmid

#61566

Purpose

Standardized mini-Tn5 delivery plasmid, gentamicin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV2_PrME

Plasmid

#207264

Purpose

Mammalian expression of Dengue virus 2 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV2_PrME (POLY Dengue virus 2)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV3_PrME

Plasmid

#207265

Purpose

Mammalian expression of Dengue virus 3 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV3_PrME (POLY Dengue virus 3)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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