We narrowed to 13,712 results for: cas9

1. pX330-U6-W308_guide-CBh-hSpCas9

   Plasmid

   #188546

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-U6-L101_guide-CBh-hSpCas9

   Plasmid

   #188547

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

   Plasmid

   #222990

   Purpose

   Bacterial expression of iGeoCas9(C1) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
   

   Use

   CRISPR

   Tags

   CL7, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3-SauriCas9-KKH-BlastR

   Plasmid

   #220972

   Purpose

   Expresses human codon-optimized SauriCas9-KKH

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SauriCas9-KKH
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-dCas9-BFP-KRAB

   Plasmid

   #221502

   Purpose

   CRISPRi machinery fish codon optimized

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   D10A and H839A point mutations

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mPLD3-sgRNA-Cas9-mcherry

   Plasmid

   #199700

   Purpose

   encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

   Depositor

   David Nemazee

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   P2A-RFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF-SpCas9D10A nickase

   Plasmid

   #216736

   Purpose

   Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF-1-alpha core

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AID-C12*-dCas9-BE4max

   Plasmid

   #216731

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-dCas9-BE4max (AICDA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-SpCas9HF1-P2A-puro

   Plasmid

   #214357

   Purpose

   Expresses SpCas9HF1 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9HF1 and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SlugCas9-P2A-puro

    Plasmid

    #214353

    Purpose

    Expresses SlugCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF823_EFS-Cas9-P2A-Puro

    Plasmid

    #211645

    Purpose

    EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCF226_EFS-Cas9-P2A-Puro

    Plasmid

    #211644

    Purpose

    EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

    Plasmid

    #211821

    Purpose

    PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter, U6 promoter and hPGK pr…

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cas9-T2A-H2B mCitirine

    Plasmid

    #210471

    Purpose

    Coexpression of Cas9 and H2B mCitrine

    Depositor

    Amro Hamdoun

    Insert

    Cas9-T2A-H2B mCitirine
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mCitrine on C terminal of H2B

    Expression

    Mammalian

    Mutation

    Cas9 contains 3xFLAG tag and nuclear localization…

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. eA3Ai-Cas9n-UGI-NLS

    Plasmid

    #207165

    Purpose

    Express A3A with an N57G point mutation and an intron in a BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
    

    Use

    Tags

    Cas9-UGI--NLS

    Expression

    Mammalian

    Mutation

    N57G

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-HypaSpCas9-NLS-6xHis

    Plasmid

    #207384

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-B-HeFSpCas9-NLS-6xHis

    Plasmid

    #207392

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-HeFSpCas9-NLS-6xHis

    Plasmid

    #207391

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PET-evoSpCas9-NLS-6xHis

    Plasmid

    #207389

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDL25_Cas9-His Δcys E1207C

    Plasmid

    #206286

    Purpose

    Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E1207C

    Promoter

    T7

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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