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pYO765

Plasmid

#235762

Purpose

Expression of ScVPS38 delta C2 (1-154) -EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Use

Tags

EGFP

Expression

Yeast

Mutation

aa 1-154 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO766

Plasmid

#235763

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Use

Tags

EGFP

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGM266

Plasmid

#220180

Purpose

Expresses exogenous TPR mutant TTC1 (R190A_R194A) with an N-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

sfGFP

Expression

Mutation

R190A + R194A

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGM268

Plasmid

#220181

Purpose

Expresses exogenous C-terminal mutant TTC1 (I271D) with an N-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

sfGFP

Expression

Mutation

I271D

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGM265

Plasmid

#220179

Purpose

Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

sfGFP

Expression

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR/eCas9-GPIDAF

Plasmid

#213710

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

CRISPR

Tags

3XFLAG and Twin-strep-tag

Expression

Mutation

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR/eCas9-GPIBY55

Plasmid

#213709

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

CRISPR

Tags

3XFLAG and Twin-strep-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS R71/75E C396/7A-HA

Plasmid

#130920

Purpose

Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E C396/7A

Use

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate; aa 396 and 397…

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-TIRAP-cGASΔN-HA

Plasmid

#130921

Purpose

Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

TIRAP-ΔNcGAS

Use

Tags

HA

Expression

Mammalian

Mutation

cGAS N terminus (aa1-159) replaced with the N ter…

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Fyn-cGASΔN-HA

Plasmid

#130922

Purpose

Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

Fyn-ΔNcGAS

Use

Tags

HA

Expression

Mammalian

Mutation

cGAS N terminus (aa1-159) replaced with the dual …

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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