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Plasmid#85808PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionPlantMutationPromoterAtRPS5AAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabePuro-bcl2
Plasmid#21144DepositorInsertpBabePuro-bcl 2
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKM464 (deletions)
Plasmid#108322PurposeOrbit integrating plasmid to delete target gene.DepositorInsertBxb1 attB site
UseOrbit integration plasmid for creating knockouts …TagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKIR1.1
Plasmid#85758PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporterDepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAGExpressionPlantMutationPromoterAtRPS5AAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL-pRc/CMV
Plasmid#19999DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationwild typePromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIOM15
Plasmid#20869DepositorInsertaphA::aadA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188
Plasmid#67296PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-GFPuv
Plasmid#106382PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM
Plasmid#12247Purpose2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmidDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMKV059
Plasmid#133314PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPTDepositorInsertLuc+, IPT
UseTagsExpressionPlantMutationPromoterAtUbi10, 35SAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMo130-TelR
Plasmid#50799PurposeSuicide vector for generating chromosomal deletions in multidrug-resistant bacteriaDepositorInserttellurite-resistance cassette
UseSuicide vectorTagsExpressionMutationPromoterPCs12Available sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG79-attL1-pegRNA-attL2
Plasmid#213050PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBb(B5)1k-GFPuv
Plasmid#106393PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1-B5 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#167547PurposeUsed for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.DepositorInsertschloramphenicol acetyl transferase
pUC19 origin of replication
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-J23107-GFPuv
Plasmid#106389PurposepBBR1k-GFPuv with Anderson promoter J23107 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCpfSA
Plasmid#187890PurposeCRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1322 HPV-16 E6
Plasmid#8642DepositorInsertHPV-16 E6 (E6 Human papillomavirus)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCAS9-mCherry-Frame +2
Plasmid#66941PurposeCRISPaint frame selector +2DepositorInsertgRNA frame +2
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-K560-24xGCN4_v4
Plasmid#72229PurposeA fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide arrayDepositorInsertK560 kinesin
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1-N-mCherryTag
Plasmid#218186PurposeTo tag Hevin with mCherry at the N-terminalDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMKV058
Plasmid#133313PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2DepositorInsertLuc+. WUS2
UseTagsExpressionPlantMutationPromoterAtUbi10, NosAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-VP16AD
Plasmid#26259DepositorInsertVP16AD-ZIP+
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3HA-TRP1
Plasmid#41599DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTags3xHA and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-dCas9
Plasmid#218371PurposeExpression of dCas9 for CRISPR-interferenceDepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationPromoterPtetAAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro
Plasmid#60909PurposeA minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imagingDepositorInsertK560-E236A
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbE0k-RFP
Plasmid#35365DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSFVCs-lacZ
Plasmid#92076PurposeThis is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.DepositorInsertC-lacZ
UseC-lacz expression plasmid, for run-off transcript…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVCT-tTR-KRAB
Plasmid#11643PurposeTet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCHW111117
Plasmid#222460PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber mosaic virus)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-strong
Plasmid#108313PurposeGFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterpFAB4026Available sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-13Myc-TRP1
Plasmid#41601DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTags13Myc and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRPLC
Plasmid#122261PurposepBRBAD-rbsD-RFP-B0015- B0015-LasR-rbs34-J23108-pBRlasI-rbsD-RFP- B0015DepositorInsertpBRBAD, pBRlasI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBb(RK2)1k-GFPuv
Plasmid#106394PurposeIPTG-inducible trc promoter expressing gfpuv on RK2 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHW110035
Plasmid#222444PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.DepositorInsertLoxP-mCherry-stop
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ZFN-AAVS1
Plasmid#89707PurposeMammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locusDepositorInsertZFN-AAVS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORI28
Plasmid#71595PurposeEC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.DepositorTypeEmpty backboneUseIntegration vector for bacteriaTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-GFP/cre
Plasmid#49054PurposeRetroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinaseDepositorInsertGFP/Cre
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDonor MCS Rosa26
Plasmid#37200DepositorInsertsRosa26 left targeting arm
MCS
Rosa26 right targeting arm
UseGenomic targeting - zinc fingerTagsExpressionMutationPromoterAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKC96 pBABE H2B-mCherry
Plasmid#192695PurposeRetroviral transgene expression of human H2BDepositorInsertH2B
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE515
Plasmid#149288PurposeT-DNA encoding TRV2 complete genome with MCSDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbB5c-GFP
Plasmid#35353DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-HHARI
Plasmid#17450DepositorInsertHHARI (ARIH1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCHW111101
Plasmid#222452PurposeLevel 1, Position 7 containing CBS4-miniDFR::Cre::NOS.DepositorInsertCBS4-miniDFR::Cre::NOS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPTg.T2FpAXK (#52)
Plasmid#35962DepositorInsertsTie2 promoter
SV40 pA
Tie2 enhancer
UseTagsExpressionMutationPromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUPRT::DHFR-D
Plasmid#58528Purposereplace TgUPRT with DHFR by homologous recombinationDepositorInsertDHFR cassette
UseGateway cloningTagsExpressionMutationpyrimethamine resistantPromoterDHFRAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH776_pJH_LldR_PlldR-sfGFP.3opal
Plasmid#163735PurposeLactate-inducible expression of green fluorescent protein encoding three opal codonsDepositorInsertlldr gfp.3TGA
UseTagsExpressionBacterialMutationgfp with first three leucine codons mutated to am…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH726_pJH_LldR_PlldR-sfGFP.15TAG
Plasmid#163730PurposeLactate-inducible expression of green fluorescent protein encoding fifteen amber codonsDepositorInsertlldr gfp.15TAG
UseTagsExpressionBacterialMutationgfp with first 15 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDZ415 (24MS2SL loxP-Kan-loxP)
Plasmid#45162DepositorInsert24xMS2 Stem Loop Cassette
UseTagsExpressionMutationPromoterAvailable sinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL3-pegRNA-attL2
Plasmid#213052PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only