We narrowed to 2,291 results for: Kap

1. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

   Plasmid

   #177334

   Purpose

   bacterial expression of active SARS-CoV-2 3C-like proteinase

   Depositor

   Christopher Overall

   Article

   Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
   

   Use

   Tags

   Factor Xa site-3xFlag-Myc-6xHis

   Expression

   Bacterial

   Mutation

   silent mutation C to T at position 10546 (elimina…

   Promoter

   T7

   Available Since

   Nov. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDONR223_HSD17B10_WT_V5

   Plasmid

   #82941

   Purpose

   Gateway Donor vector containing HSD17B10, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   HSD17B10 (HSD17B10 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-PDGFRb559-562del-IRES2-EGFP

   Plasmid

   #204355

   Purpose

   Expresses a mutant PDGFRb gene (559-562 deletion).

   Depositor

   Hirofumi Nakatomi

   Article

   Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

   Insert

   Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
   

   Use

   Tags

   Expression

   Mutation

   559-562 deletion

   Promoter

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CMV-PDGFRb559-562del-HA

   Plasmid

   #204351

   Purpose

   Expresses a mutant PDGFRb gene (559-562 deletion). Used for DNA delivery using Adeno-associated Virus.

   Depositor

   Hirofumi Nakatomi

   Article

   Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

   Insert

   Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   559-562 deletion

   Promoter

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

   Plasmid

   #224697

   Purpose

   Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
   

   Use

   Tags

   Gly-Gly-6xHis

   Expression

   Bacterial

   Mutation

   silent mutation C to T at position 10546 (elimina…

   Promoter

   T7

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

   Plasmid

   #213026

   Purpose

   Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

   Plasmid

   #213030

   Purpose

   Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

   Plasmid

   #213031

   Purpose

   Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

   Depositor

   Martin S. Taylor, Donna Romero

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
   

   Use

   Luciferase

   Tags

   Firefly luciferase antisense intron (3' UTR)

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE bidirectional tet-on

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Use

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid

    #177335

    Purpose

    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

    Depositor

    Christopher Overall

    Article

    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Factor Xa site-3xFlag-Myc-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid

    #224698

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FGFR2 3xFlag

    Plasmid

    #110063

    Purpose

    FGFR2 expression in mammalian cells (JCOPCO paper)

    Depositor

    Martin Fernandez-Zapico

    Article

    Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

    Insert

    fibroblast growth factor receptor 2 (FGFR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FGFR2 F276C 3xFlag

    Plasmid

    #110064

    Purpose

    FGFR2 F276C expression in mammalian cells (JCOPO paper)

    Depositor

    Martin Fernandez-Zapico

    Article

    Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

    Insert

    fibroblast growth factor receptor 2 (FGFR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mutated Phenylalanine 276 to Cysteine (F276C)

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FGFR2 K41E 3xFlag

    Plasmid

    #110105

    Purpose

    expresses FGFR2 with a single amino acid change in mammalian cells

    Depositor

    Martin Fernandez-Zapico

    Article

    Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

    Insert

    fibroblast growth factor receptor 2 (FGFR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mutated Lysine 41 to Glutamic acid (K41E)

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PA-GFP-α-Tubulin

    Plasmid

    #213900

    Purpose

    Imaging of Tubulin using Photoactivatable GFP

    Depositor

    Lukas Kapitein

    Insert

    PA-GFP-α-Tubulin
    

    Use

    Tags

    Photoactivatable GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET12a-HAL

    Plasmid

    #47097

    Depositor

    Marina Ramirez-Alvarado

    Insert

    HAL
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET11a-p65(19-325)

    Plasmid

    #44744

    Depositor

    Gourisankar Ghosh

    Insert

    p65 (Rela Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AA 19-325

    Promoter

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only