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We narrowed to 7,952 results for: Lif

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Showing: 2001 - 2020 of 7952 results

  1. mE-mCherry2A

    Plasmid
    #236248
    Purpose
    Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    mCherry2A
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence to mCherry with no stop …
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236248-plasmid-map-sequence-id-486041

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  2. mE-GFP2A

    Plasmid
    #236247
    Purpose
    Middle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    eGFP
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence with no stop codon
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236247-plasmid-map-sequence-id-486040

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  7. pDUP2

    Plasmid
    #223517
    Purpose
    cogfp copy 1 under control of Ptet, cogfp copy 2 under control of Ptac
    Depositor
    Yolanda Schaerli
    Article
    Mihajlovic et al eLife. 2025 Apr 2;13:RP97216. doi: 10.75
    Inserts
    CoGFP
    CoGFP (second copy)
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    anti-dimerization mutations S129G, 22 C154S, D156…
    Promoter
    Ptac, Ptac and Ptet, and Ptet
    Available Since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    223517-plasmid-map-sequence-id-444179

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  17. RNF169-Halo HRD

    Plasmid
    #207083
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207083-plasmid-map-sequence-id-432319

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  18. pCL1315

    Plasmid
    #217957
    Purpose
    Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    F84A, L92A, Y99A
    Promoter
    CMV
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217957-plasmid-map-sequence-id-430211

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  19. pCL1314

    Plasmid
    #217956
    Purpose
    Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between A95 and C96
    Promoter
    CMV
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217956-plasmid-map-sequence-id-430210

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  20. pINER3R

    Plasmid
    #185883
    Purpose
    In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    185883-plasmid-map-sequence-id-430023

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Showing: 2001 - 2020 of 7952 results