We narrowed to 2,021 results for: SP6

1. pJK048

   Plasmid

   #75230

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and n/a

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-EGFP-eDHFR(69K6)-MCS

   Plasmid

   #172100

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-20aa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMV361_MjTyrRS

   Plasmid

   #193250

   Purpose

   Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

   Depositor

   Jessica Seeliger, Peter Schultz

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Insert

   MjTyrRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hsp60

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-MCS-eDHFR(69K6)-EGFP

   Plasmid

   #172101

   Purpose

   Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   22aa-eDHFR(69K6)-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AdMKP-3 S197A

   Plasmid

   #131522

   Purpose

   Expresses MKP-3 with S197A mutation in mammalian cells

   Depositor

   Haiyan Xu

   Article

   Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

   Insert

   Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Expression

   Mutation

   changed Serine 197 to Alanine

   Promoter

   CMV

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mouse c-myc (exons II and III)

   Plasmid

   #8448

   Depositor

   Frederic Mushinski

   Article

   Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

   Insert

   Mouse c-myc (exons II and III) (Myc Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Exons II and III only

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

7. AdMKP-3 S159A

   Plasmid

   #131520

   Purpose

   Expresses MKP-3 with S159A mutation in mammalian cells

   Depositor

   Haiyan Xu

   Article

   Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

   Insert

   Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Expression

   Mutation

   changed Serine 159 to Alanine

   Promoter

   CMV

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]

   Plasmid

   #124068

   Purpose

   CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)

   Depositor

   Gregor Bucher

   Article

   He et al Elife. 2019 Oct 18;8. pii: 49065. doi: 1

   Insert

   eb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Insect

   Mutation

   Promoter

   bhsp68

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AdMKP-3 S159AS197A

   Plasmid

   #131523

   Purpose

   Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

   Depositor

   Haiyan Xu

   Article

   Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

   Insert

   Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Expression

   Mutation

   changed Serine 159 to Alanine and Serine 197 to A…

   Promoter

   CMV

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. rAAV2-retro helper

    Plasmid

    #81070

    Purpose

    retrograde AAV helper vector, carrying rep and modified capsid gene

    Depositor

    David Schaffer, Alla Karpova

    Article

    Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

    Inserts

    rep
    rAAV2-retro cap
    

    Use

    AAV and Unspecified; Helper vector

    Tags

    Expression

    Mutation

    Pro2Ala, possibly Asp613Gly and changes made to A…

    Promoter

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. IP6K2 gRNA (BRDN0001145795)

    Plasmid

    #77817

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IP6K2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IP6K2 (IP6K2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. IP6K2 gRNA (BRDN0001145457)

    Plasmid

    #77818

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IP6K2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IP6K2 (IP6K2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IP6K2 gRNA (BRDN0001145651)

    Plasmid

    #77819

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IP6K2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IP6K2 (IP6K2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IP6K3 gRNA (BRDN0001147242)

    Plasmid

    #77655

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IP6K3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IP6K3 (IP6K3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IP6K3 gRNA (BRDN0001147705)

    Plasmid

    #77656

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IP6K3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IP6K3 (IP6K3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IP6K3 gRNA (BRDN0001147058)

    Plasmid

    #77654

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IP6K3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IP6K3 (IP6K3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IP6K2 gRNA (BRDN0001146075)

    Plasmid

    #77816

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IP6K2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IP6K2 (IP6K2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSMT-MjtRNA-GFP(47TAG)

    Plasmid

    #193251

    Purpose

    Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

    Depositor

    Jessica Seeliger, Peter Schultz

    Article

    Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

    Inserts

    GFP(47TAG)
    MjtRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon for Tyr47 replaced with amber stop codon TA…

    Promoter

    hsp60 and metU

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-IHPK3

    Plasmid

    #23449

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    IHPK3 (IP6K3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    MMs?

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pWZL Neo Myr Flag IHPK2

    Plasmid

    #20538

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IHPK2 (IP6K2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only