We narrowed to 2,318 results for: pET28

1. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Use

   Tags

   C-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RpHLuorin2

   Plasmid

   #180227

   Purpose

   for bacterial expression of recombinant ratiometric pHluorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2
   

   Use

   Tags

   His-tag followed by thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC28-Bsa4

   Plasmid

   #26103

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SpyTag003-sfGFP

   Plasmid

   #133454

   Purpose

   Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-sfGFP
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SpyTag003-mKate2

   Plasmid

   #133452

   Purpose

   Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mKate2
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NW50

   Plasmid

   #133445

   Purpose

   Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW50
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MYST3

   Plasmid

   #25181

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MYST3_13 (KAT6A Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains catalytic region ( residues 497-780).

   Promoter

   Available since

   Oct. 25, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

8. NW30

   Plasmid

   #133444

   Purpose

   Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW30
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDR119

   Plasmid

   #13332

   Depositor

   Martin Gellert

   Article

   Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

   Insert

   DNA ligase IV (LIG4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pJBL715

    Plasmid

    #140385

    Purpose

    Expresses MphR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-MphR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJBL723

    Plasmid

    #140393

    Purpose

    Expresses HucR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-HucR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJBL713

    Plasmid

    #140383

    Purpose

    Expresses CtcS protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CtcS-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Human USP30 (64-502, construct 8, MG-26-21)

    Plasmid

    #110745

    Purpose

    Bacterial expression of human USP30 (construct 8)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    F348D, M350D, I353E + insertion deletion: 64-357;…

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SurA

    Plasmid

    #213138

    Purpose

    Expresses chaperone SurA

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SurA (21-428) (surA E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG247 His-iFAST

    Plasmid

    #130808

    Purpose

    Expresses His-tagged iFAST in bacteria

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

    Insert

    iFAST
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET16_Caspase-4_p10

    Plasmid

    #214308

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Human RNF144A (16-228)

    Plasmid

    #110752

    Purpose

    Bacterial Expression of RNF144A RBR domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    RNF144A (RNF144A Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tUI-HA

    Plasmid

    #122662

    Purpose

    Express tUI-HA protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tUI-HA
    

    Use

    Tags

    HA-tag and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. SpyTag003-mClover3

    Plasmid

    #133453

    Purpose

    Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-mClover3
    

    Use

    Tags

    GSGSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJBL701

    Plasmid

    #140371

    Purpose

    Expresses TetR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-TetR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge