We narrowed to 21,911 results for: tes

1. pCMV HA hRb delta CDK

   Plasmid

   #58906

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-neo:Beta2meGFP

   Plasmid

   #45096

   Depositor

   Henry Lester

   Article

   Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

   Insert

   nAChR beta2 (Chrnb2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   There are strain differences between C57BJ/6 and …

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. mVenus-proα1(I)

   Plasmid

   #119845

   Purpose

   Expresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α1 chain (Col1a1 Mouse)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Col1a1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-RaichuEV-Rac1(Y40C)

   Plasmid

   #164903

   Purpose

   Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   RaichuEV-Rac1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y40C mutation in the Rac1 domain whcih make the s…

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRESh-GFPII-SM-Dlk1

   Plasmid

   #108932

   Purpose

   GFP-tagged vector expressing full length isoform of Dlk1

   Depositor

   Anne Ferguson-Smith

   Article

   Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

   Insert

   Dlk1 (Dlk1 Mouse)
   

   Use

   Tags

   GFPII

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. BII-BCA-Prom-ASCL2

   Plasmid

   #133394

   Purpose

   Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

   Depositor

   Blair Madison

   Article

   Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

   Insert

   destabilized tdTomato-NLS and destabilized NLS-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free) drives expressi…

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCerulean-proα1(I)

   Plasmid

   #119842

   Purpose

   Expresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α1 chain (Col1a1 Mouse)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Col1a1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST17-ALDH1A3

   Plasmid

   #189750

   Purpose

   The construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 (ALDH1A3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE GFP-Ago2 S387A

   Plasmid

   #60654

   Purpose

   GFP-Ago2 S387A mutant lentiviral vector

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   S387A

   Promoter

   EF1a

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. mApple-proα2(I)-L

    Plasmid

    #120161

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Flag-RNF168 delta RING

    Plasmid

    #133982

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of RING

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLVX-Flag-Polß(WT)-IRES-puro

    Plasmid

    #128653

    Purpose

    mammalian expression of Flag-Polß(WT) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    CMV

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR1A-HA-Jag1

    Plasmid

    #46052

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Jag1 (Jag1 Rat)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pDEST17-ALDH1A1

    Plasmid

    #189749

    Purpose

    The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A1 (ALDH1A1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mApple-proα1(I)

    Plasmid

    #119844

    Purpose

    Expresses mouse Type I procollagen α1 chain (Col1a1) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α1 chain (Col1a1 Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Col1a1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBpuro_KLF4_ER

    Plasmid

    #34609

    Depositor

    Michael Ruppert

    Article

    Foster et al Oncogene. 2005 Feb 24;24(9):1491-500.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Retroviral

    Tags

    Myc epitope tag and estrogen receptor fusion

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-Sec22b-P33-shR

    Plasmid

    #208359

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations (shRNA resistance), 33 aa p…

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4-His-Max-A-PQBP1

    Plasmid

    #25802

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

    Insert

    PQBP1 (PQBP1 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pGEX 4T-1 GST-Ago2 S387A

    Plasmid

    #60656

    Purpose

    Expresses GST-Ago2 S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S387A

    Promoter

    tac

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Flag-RNF168 delta MIU1

    Plasmid

    #133978

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of MIU1

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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