We narrowed to 6,904 results for: crispr cas9 plasmids

1. pLenti-nSpCas9-BEACON2

   Plasmid

   #209041

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   BEACON2-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-nSpCas9-evoCDA1

   Plasmid

   #209042

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   evoCDA1-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-nSpCas9-TadA8.20m

   Plasmid

   #209045

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8.20m-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-3xHA-dSpCas9

   Plasmid

   #201953

   Purpose

   Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dSpCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A on SpCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. modRNAc0-Cas9-2A-GFP

   Plasmid

   #170181

   Purpose

   IVT template plasmid for making modified Cas9GFP RNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   Cas9-2A-GFP
   

   Use

   Ivt template plasmid

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. modRNAc0-Cas9-2A-Puro

   Plasmid

   #172855

   Purpose

   IVT template plasmid for making modified Cas9Puro RNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   Cas9-2A-Puro
   

   Use

   Ivt template plasmid

   Tags

   2A-Puro

   Expression

   Mutation

   Promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

   Plasmid

   #121826

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/Dnmt3a-3L (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

   Plasmid

   #83306

   Purpose

   Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

   Depositor

   Tohru Kimura

   Article

   Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

   Insert

   sgRNA and dCas9 from pX330
   

   Use

   Lentiviral

   Tags

   FLAG and PA tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA and CBh for dCas9

   Available since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

   Plasmid

   #159086

   Purpose

   Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

   Depositor

   Jeremy Day

   Article

   Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

   Insert

   empty crRNA-acRNA backbone, dCas9-P2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hSYN, U6

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113687

    Purpose

    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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