We narrowed to 4,881 results for: phy

1. pAG8Ha-EWS_RRM-RGG(359-513)

   Plasmid

   #188046

   Purpose

   Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-358, 514-656

   Promoter

   T7

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEmal10H-MBP-EWSR1-449-513

   Plasmid

   #199439

   Purpose

   Expresses RG2 (the second RG-rich region, 449-513) of EWS

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-448, 514-656

   Promoter

   T7

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-CMV-scFVDnmt3A_bGHpA

   Plasmid

   #177349

   Purpose

   AAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   598–908 aa of mouse Dnmt3a

   Promoter

   CMV

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-CMV-scFVDnmt3L_bGHpA

   Plasmid

   #177350

   Purpose

   AAV expression of scFV-fused C terminal domain of Dnmt3l for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   194–415 aa

   Promoter

   CMV

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-hSyn-scFVtet2_bGHpA

   Plasmid

   #177353

   Purpose

   AAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 2 (TET2 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   Catalytic domains of human TET2 (1129–1936 and 14…

   Promoter

   human Synapsine promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-TRE-scFVDnmt3L_bGHpA

   Plasmid

   #177356

   Purpose

   AAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   194–415 aa

   Promoter

   CMV promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-TRE-scFVtet2_bGHpA

   Plasmid

   #177358

   Purpose

   AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 2 (TET2 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   Catalytic domains of human TET2 (1129–1936 and 14…

   Promoter

   Tetracycline-dependent promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LMPd Amt PHGDH

   Plasmid

   #209409

   Purpose

   Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

   Depositor

   Russell Jones

   Article

   Ma et al Immunity. 2019 Nov 19;51(5):856-870.e5.

   Insert

   Phgdh shRNA (Phgdh Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hKCNQ5(K289N).ires.GFP-pcDNA3

   Plasmid

   #204364

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   KCNQ5 (KCNQ5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K289N

   Promoter

   CMV/T7 RNApol

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hKCNQ5(P369T).ires.GFP-pcDNA3

    Plasmid

    #204365

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P369T

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hKCNQ5(R443Q).ires.GFP-pcDNA3

    Plasmid

    #204366

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R443Q

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hKCNQ5(H576R).ires.GFP-pcDNA3

    Plasmid

    #204367

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H576R

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hKCNQ5(V145G).ires.GFP-pcDNA3

    Plasmid

    #204368

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V145G

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hKCNQ5(L341I).ires.GFP-pcDNA3

    Plasmid

    #204369

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L341I

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hKCNQ5(P369R).ires.GFP-pcDNA3

    Plasmid

    #204370

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P369R

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hKCNQ5(S448I).ires.GFP-pcDNA3

    Plasmid

    #204371

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S448I

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hKCNQ5(R178X).ires.GFP-pcDNA3

    Plasmid

    #204372

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R178X

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hKCNQ5(R443X).ires.GFP-pcDNA3

    Plasmid

    #204373

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R443X

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Myc-KLC1 delta Tail

    Plasmid

    #166965

    Purpose

    Expresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KLC1 (Klc1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    KLC1 (1-495aa)

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-KIF5AR280H

    Plasmid

    #166957

    Purpose

    Expresses FLAG-tagged KIF5A R280H protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A-R280H (Kif5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A-R280H

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-9

    Plasmid

    #129061

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA9 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-10

    Plasmid

    #129062

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA10 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-11

    Plasmid

    #129063

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA11 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-12

    Plasmid

    #129064

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA12 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-2

    Plasmid

    #129054

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA2 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-3

    Plasmid

    #129055

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA3 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-4

    Plasmid

    #129056

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA4 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-5

    Plasmid

    #129057

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA5 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA5 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-6

    Plasmid

    #129058

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA6 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-7

    Plasmid

    #129059

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA7 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-8

    Plasmid

    #129060

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA8 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

    Plasmid

    #129040

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA12 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid

    #129042

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA2targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA2 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

    Plasmid

    #129033

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA5 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA5 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid

    #129034

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA6 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

    Plasmid

    #129035

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA7 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

    Plasmid

    #129037

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA9 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid

    #129038

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA10 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid

    #129039

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA11 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid

    #129030

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA2 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge