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We narrowed to 322 results for: phy

Showing: 1 - 20 of 322 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...Our Special Collections Pages Duchenne muscular dystrophy: The most common inherited disease The genetic...genetic disease targeted is Duchenne muscular dystrophy (DMD), an X-linked recessive disorder affecting approximately...symptoms, a condition known as Becker muscular dystrophy (BMD). Dystrophin’s size makes it difficult to...Exon skipping strategies for Duchenne muscular dystrophy. gRNAs can be designed to flank a single or multiple...Kaiser J. CRISPR helps heal mice with muscular dystrophy. Science. 2015 Dec 31. doi:10.1126/science.aae0169...dystrophin expression in a mouse model of muscular dystrophy. Science. 2015 Dec 31. pii: aad5725. PubMed PMID...function in a mouse model of Duchenne muscular dystrophy. Science. 2015 Dec 31. pii: aad5143. PubMed PMID...

  2. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

    Type
    Blog Post
    ... plasmids these days. via GIPHY   You can see for yourself at giphy.com/addgene. But that’s not all... your lab if you use a GIF! via GIPHY   So head over to our GIPHY page to check out everything we’...the deal with looping GIFs? We have answers! via GIPHY   We’ve been working hard to create the most...collecting some of our favorite (moving) images on a GIPHY page! The best part is, we’re not only sharing them...   Believe us...we know the feeling. via GIPHY   Or maybe your experiment went so well you ...you after watching Addgene’s protocol videos. via GIPHY   If you’re also looking for that perfect GIF...Blugene is so excited they can’t stop dancing! via GIPHY   Don’t forget to check in regularly to see ...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... H. Siedentopf, and R,. Zsigmondy.  (1902) Ann. Phys.  315, 1 – 39.
 A. H. Voie, D. H. Burns, F. A. Spelman...embryos were obtained by using optical projection tomography (OPT)  (12) and later Hans-Ulrich Dodt’s lab ....janelia.org/lab/keller-lab http://www.physikalischebiologie.de http://www.lavisionbiotec.com/ultramicroscope-overview.html...

  5. Optogenetics Guide

    Type
    Guide
    ...light. 740–780 Phy–PIF Light-controlled Phytochrome Interacting Factor 6 (PIF6) to PhyB (Phy) interaction...utilizing FAD) domains. 441 Phytochromes BphP1–PpsR2 Bacterial phytochrome BphP1 and its natural partner...Optogenetics has been broadly applied to study the physiology of the brain and nervous system to better map...ChR1 because ChR2 has higher conductance at physiological pH and trafficks well to the membrane. Excitatory...species include: GtACR1 and GtACR2 (from the cryptophyte Guillardia theta ). Other feature enhancements...spatial and temporal control of protein activity. Phytochrome, cryptochrome, and LOV (light oxygen voltage)...domain, allowing light to activate transcription. Phytochromes and light oxygen voltage (LOV) domains function...

  6. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ... SYBR Green Technology Use qPCR to measure the physical titer of your adeno-associated virus preparation...AAV Titration by ddPCR Use ddPCR to measure the physical titer of your adeno-associated virus preparation...Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation Antibodies...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Metal Ions Small Molecules Neurotransmitters Cell Physiology Browse All You may also like... Addgene Blog:...of Cells for multimodal recordings of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881. Adam Cohen...Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide. Front Cell Neurosci. 2021 Jun...calcium microdomain in the endoplasmic reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium Fluorescent...YFP-derived sensor for measuring Cl- concentration in physiological range A genetically-encoded YFP sensor with ...of Cells for multimodal recordings of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881 Adam Cohen...

  8. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...pComb3xss anti-Gephyrin nanobody GC1 Gephyrin Mouse Llama 134721 GC83 pComb3xss anti-Gephyrin nanobody GC83...pComb3xss anti-Gephyrin nanobody GC92 Gephyrin Human Llama 145827 GS8 pComb3xss anti-Gephyrin nanobody GS8...GS8 Gephyrin Human Llama 145828 GS41 pComb3xss anti-Gephyrin nanobody GS41 Gephyrin Human Llama 145829...channel pS586 Rat Mouse IgG2a 182100 Anti-Gephyrin [L106/83R] Gephyrin Human Mouse IgG2a 182102 Anti-AMIGO-...Pan-Kvbeta K+ channel Mouse IgG2a 188192 Anti-Gephyrin [L106/22R] Gephyrin Human Mouse IgG2a 188193 Anti-Cav1.2... channel Rat Mouse IgG2a 199397 Anti-Gephyrin [L106/23R] Gephyrin Human Mouse IgG2a 199398 Anti-Gamma-...] QKI-7b Human Mouse IgG2a 206541 Anti-PhyH/PAHX [N210/5R] PhyH/PAHX Human Mouse IgG2a 206542 Anti-Lhx6.1...

  9. AAV Molecular Tools

    Type
    Collection
    ..., Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments...Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators ...Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP for labeling of axon terminals. 1 Zeng ...Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression ...expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals. 1 Luo 60658 pAAV-EF1α-...of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato 8 Scanziani Tools for Cell...Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6 Zhang Don’t See What You...

  10. Cre-lox system

    Type
    Collection
    ...131781 pCS2+PhyBΔCreC CreLite - Red light-inducible Cre; C terminus Cre fused to PhyBdelta CMV Mammalian...131782 pME-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit...131785 pAAV-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector Cbh...131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral...Chicken cardiac troponin T AAV Pu 134308 pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre Cre-ERT2 - Tamoxifen ...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre...

  11. Retrograde AAV viral preps

    Type
    Collection
    ...55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Deisseroth...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Deisseroth...AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting ...-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium...AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting ...

  12. Bacterial Expression Systems

    Type
    Collection
    ...from the culture and purified through affinity chromatography. To facilitate the purification process, proteins...68940 pRMC2 Pxyl/TetO Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267 pBAD33.1 pBAD Arabinose...glutamicum Timothy Lu 36252 pRibo EcoHind Riboswitch Teophylline Escherichia coli , Mycobacterium smegmatis Carolyn...Jessica Seeliger 36251 pRibo BsaHind Riboswitch Teophylline Escherichia coli , Mycobacterium smegmatis Carolyn...

  13. Microbiology Resources

    Type
    Collection
    ... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains... Rickettsia sp. Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Cas9 orthologs have also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and ...immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun . 439(1):132-6. PMID: 23942116 Gilbert...Zhang F. 2015. In vivo genome editing using Staphylococcus aureus Cas9. Nature . 520(7546):186-91. PMID...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...72286 pCI SMN1 SMN1 CMV Spinal muscular atrophy Elliot Androphy 72873 PMXS-SLC1A3 SLC1A3 Episodic ataxia...Riggs 37057 GFP-SMN SMN1 GFP CMV Spinal muscular atrophy Greg Matera 38005 pET-21a(+)/MBP-His-PINK1 PINK1...Schmitt-Ulms 61946 4QQ6 SMN1 His T7 Spinal muscular atrophy Cheryl Arrowsmith 65106 pcDNA3-ADAM10-HA ADAM10...SMN1 myc HIS SMN1 His, Myc CMV Spinal muscular atrophy Gideon Dreyfuss 71875 pET-Sac-Abeta(M1-42) APP ...156022 SMN1-1 SMN1 V5-MCP EF1a Spinal muscular atrophy Eugene Yeo 156073 TARDBP TARDBP V5-MCP EF1a ALS...178154 COQ2_Halo_N_allele COQ2 Halo Multiple system atrophy Michael Ward 178155 DAO_Halo_C_allele DAO Halo ... Dystonia-parkinsonism, Infantile neuroaxonal dystrophy Michael Ward 178167 RNF216_Halo_C_allele RNF216...

  16. Ras Pathway

    Type
    Collection
    ... removal of a phosphate group. Under normal physiological conditions GTPases play prominent roles in signaling...Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-95. PubMed PMID: 17428555...

  17. CRISPR Guide

    Type
    Collection
    ... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus...Zhang, F. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. ...immunoprecipitation (enChIP) using CRISPR. Biochemical and Biophysical Research Communications . 439 (1), 132–136. PMID...

  18. Video Library

    Type
    Protocol
    ... AAV Titration by qPCR Use qPCR to measure the physical titer of your adeno-associated virus preparation...
Showing: 1 - 20 of 322 results