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We narrowed to 273 results for: phy

Showing: 1 - 20 of 273 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...Our Special Collections Pages Duchenne muscular dystrophy: The most common inherited disease The genetic...genetic disease targeted is Duchenne muscular dystrophy (DMD), an X-linked recessive disorder affecting approximately...symptoms, a condition known as Becker muscular dystrophy (BMD). Dystrophin’s size makes it difficult to...Exon skipping strategies for Duchenne muscular dystrophy. gRNAs can be designed to flank a single or multiple...Kaiser J. CRISPR helps heal mice with muscular dystrophy. Science. 2015 Dec 31. doi:10.1126/science.aae0169...dystrophin expression in a mouse model of muscular dystrophy. Science. 2015 Dec 31. pii: aad5725. PubMed PMID...function in a mouse model of Duchenne muscular dystrophy. Science. 2015 Dec 31. pii: aad5143. PubMed PMID...

  2. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

    Type
    Blog Post
    Published
    Aug. 17, 2021, 1:15 p.m.
    ... plasmids these days. via GIPHY   You can see for yourself at giphy.com/addgene. But that’s not all... your lab if you use a GIF! via GIPHY   So head over to our GIPHY page to check out everything we’...the deal with looping GIFs? We have answers! via GIPHY   We’ve been working hard to create the most...collecting some of our favorite (moving) images on a GIPHY page! The best part is, we’re not only sharing them...   Believe us...we know the feeling. via GIPHY   Or maybe your experiment went so well you ...you after watching Addgene’s protocol videos. via GIPHY   If you’re also looking for that perfect GIF...Blugene is so excited they can’t stop dancing! via GIPHY   Don’t forget to check in regularly to see ...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... H. Siedentopf, and R,. Zsigmondy.  (1902) Ann. Phys.  315, 1 – 39.
 A. H. Voie, D. H. Burns, F. A. Spelman...embryos were obtained by using optical projection tomography (OPT)  (12) and later Hans-Ulrich Dodt’s lab ....janelia.org/lab/keller-lab http://www.physikalischebiologie.de http://www.lavisionbiotec.com/ultramicroscope-overview.html...

  5. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...holding a physical model of the protein in your hand – you will understand the power of a physical model.... researchers use physical models of protein structure? We have been creating physical models of proteins... your hand? At 3D Molecular Designs, we create physical models of proteins using 3D printing technology...and I encourage you to hold one. Why do we need physical models of protein structure? Structural biologists... example, when Tom Steitz was presented with a physical model of his large ribosomal subunit, he kept ...graduate students, momentarily forgetting that the physical model is based on their own modeling of the protein...in 1999 with the expressed purpose of creating physical models of proteins and other molecular structures...

  6. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... you? Physical titer: the concentration of viral particles containing viral genomes. Physical titers ...facilities report the physical titer of a viral solution (Figure 2). Because physical titers are used for...circled in red) report physical titer, which is measured by qPCR.   Physical titer is typically calculated...genome. Other than a theoretical maximum value, physical titers don’t necessarily give any indication of... AAV2 has been reported to have a near-perfect physical-to-infectious particle ratio of 1:1 (Zeltner et...for recombinant AAV2, the same study reported a physical-to-infectious particle ratio of 50:1 (Zeltner ... viral preparations is defined by the ratio of physical viral particles to infectious viral particles....

  7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...the truncated Phytochrome B (PhyBΔ) and the active protein binding domain of Phytochrome B interacting...splitting Cre and fusing each half with PhyB or PIF6, phytochrome-like fusion proteins from the plant Arabidopsis...respectively. The cofactor analog, phycocyanobilin (PCB), is required for PhyBΔ to be functional. Exposure to...systems in their own research. Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been...CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID: 30819783. Light up CreLite:...red light (650 nm) and its prosthetic group PCB, PhyB and PIF6 dimerize, bringing each terminus of Cre... to red light induces PhyBΔ and PIF6APB binding, bringing the two halves of Cre together. Image from Eisenhoffer...

  8. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...University of Queensland, whose outstanding marine photography has attracted much attention over the past few...” Daniel went on to discuss his work: “Macro photography has been my passion for a while - a tool to expand...life cycle of the creature they deal with.” So photography can be used as a way to better understand and...scientist and non-scientist alike. “The other thing photography has shown to me - it requires an awful amount...data, publish in time, defend the thesis, etc. Photography is critically important in many fields of biology... can be found on his website http://www.microworldsphotography.com/. Another artist who spent his life...stated “Albert G. Richards, a teacher of dental radiography, had focused his x-ray machine not on teeth, ...

  9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...such as prototrophy, the ability to synthetize all compounds needed for growth, or auxotrophy, the inability...synthesize a compound needed to grow. Auxotrophy and prototrophy come into play during cloning if you’...lab created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy. Negative selection...plasmid backbone contains your gene of interest. Auxotrophy Besides antibiotic selection, you can select ...amino acid), and a plasmid that complements the auxotrophy (i.e., synthesizes the critical amino acid). ...

  10. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...Zhang et al., 2018) and the model bryophyte Physcomitrium (Physcomitrella) patens (Bezanilla et al., 2005...Interference. Annu Rev Biophys 42:217–239 . https://doi.org/10.1146/annurev-biophys-083012-130404 Zhang ...protein abundance of your specific target required physical isolation of the visually identified subpopulation...Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella patens with an Internal Marker for Silencing...2012) An Inducible RNA Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in...Silencing and Simplified Transgenic Screen. Plant Physiol 178:989–1001 . https://doi.org/10.1104/pp.18.00828...

  11. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...afflictions. Elia Di Schiavi - Spinal muscular atrophy Dr. Elia Di Schiavi is a Research Scientist at ...Naples, Italy. His group works on spinal muscular atrophy, a disease in which disruption of the smn1 gene...the smn1 gene is known to cause spinal muscular atrophy, the molecular mechanisms behind the disease are...restore motor neuron function in spinal muscular atrophy patients. Noushin Nabavi - Peritoneal Mesothelioma...peritoneal mesothelioma with an international team of physicians and scientists.  Although the form of malignant...of incidence and prevalence of spinal muscular atrophy (SMA)." European Journal of Paediatric Neurology...

  12. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...et. al.). Physical versus functional titer Viral titers usually come in two flavors, physical or functional...the target cell’s genome. Physical titer The two most common assays for physical titer are direct p24 measurements...functional. Physical titers measure the amount of viral particles in a sample and are usually based on the...methods tend to be more accurate than measuring physical titer, there is a risk that they may underestimate...

  13. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...can be achieved using pharmacological or electrophysiological methods (Deisseroth, 2011; Mei and Zhang...which are essential in optogenetics to study physiological and behavioral processes (Abdallah et al., 2018...of the DA neurons produce behavioral and electroencephalography evidences of arousal in mice previously...enabled the drawing of a detailed and functional topography of the neural circuit architecture of the brain... differing in pigmentation." Biochemical and biophysical research communications 78.1 (1977): 237-243....site-specific recombination." Annual review of biophysics and biomolecular structure 30.1 (2001): 87-104...

  14. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...variables - your approach, your workflow, or your physical setup - to prevent the mistake, just like you ...light-sensitive reagents” or “re-sterilize now.” Have a physical copy of the protocol when you work and make a ...time to do them carefully and thoroughly. Have a physical copy on hand as you run your experiments. Try ...your plate maps onto your plates, alongside your physical copy. If you’ve got messy handwriting, reach for...accuracy.  Bench setup Small adjustments in the physical organization of your workspace and your workflow...

  15. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...across broader physical areas of the brain and enable access to neurons that are physically blocked, such...driven by neurons. By studying how neurons are physically connected, we can begin to understand how the... have identified 180 brain regions. Both this geography and the way the brain is connected to itself across...Therefore, by simply looking at how neurons are physically connected we can begin to understand how the ...

  16. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...insensitivity of yeast strains to some antibiotics. Auxotrophy is defined as the inability of an organism to... functional gene which complements the host’s auxotrophy. When grown on media NOT containing the nutrient...and provides the element needed to overcome the auxotrophy as well as additional uses for said element. ...plasmid may be expressed at higher than normal physiological levels due to high copy numbers. This creates...the presence of the selection marker at non-physiological levels. Scientists have tried varied approaches...

  17. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and ...terminals with one vector. AAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane...tethered GFP to enhance axonal labeling and synaptophysin-tagged mRuby to enrich pre-synaptic labeling...targeted tdTomato and pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE...available in a ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic...

  18. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...developments. Model organisms often feature genetic, physiological, or developmental characteristics that make ...are great mammalian models due to their size, physiology, and genetic similarities with humans. However...However, rats have some key differences in physiology, intelligence, and human correspondence that give them...norvegicus. Image from Staffan Vilcans. Rat physiology is also easier to study and at times more closely...Biotechnology. In Escherichia coli - Recent Advances on Physiology, Pathogenesis and Biotechnological Applications...

  19. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...optogenetics: 1) electrophysiology and 2) behavioral experiments. I’m not an electrophysiologist so I cannot...numerous studies, optogenetics is used in slice physiology in order to directly show that, for example, ...behavior, you don’t necessarily need to use electrophysiology to prove that your optogenetic manipulations... (though a reviewer will probably ask for electrophysiology data too but that’s what collaborators are...

  20. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...YNP). Extremophiles - phylogenetically diverse microbes living in extreme physical and chemical conditions...a CRISPR array in the resulting Ignavibacteriae phylum genome. IgnaviCas9 is a hyperthermostable Cas9 ...lab and ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9...
Showing: 1 - 20 of 273 results