We narrowed to 32 results for: phy
-
TypeCollection...Metal Ions Small Molecules Neurotransmitters Cell Physiology Browse All You may also like... Addgene Blog:...of Cells for multimodal recordings of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881. Adam Cohen...Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide. Front Cell Neurosci. 2021 Jun...calcium microdomain in the endoplasmic reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium Fluorescent...YFP-derived sensor for measuring Cl- concentration in physiological range A genetically-encoded YFP sensor with ...of Cells for multimodal recordings of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881 Adam Cohen...
-
Trimmer Lab NeuroMab Collection
TypeCollection...pComb3xss anti-Gephyrin nanobody GC1 Gephyrin Mouse Llama 134721 GC83 pComb3xss anti-Gephyrin nanobody GC83...pComb3xss anti-Gephyrin nanobody GC92 Gephyrin Human Llama 145827 GS8 pComb3xss anti-Gephyrin nanobody GS8...GS8 Gephyrin Human Llama 145828 GS41 pComb3xss anti-Gephyrin nanobody GS41 Gephyrin Human Llama 145829...channel pS586 Rat Mouse IgG2a 182100 Anti-Gephyrin [L106/83R] Gephyrin Human Mouse IgG2a 182102 Anti-AMIGO-...Pan-Kvbeta K+ channel Mouse IgG2a 188192 Anti-Gephyrin [L106/22R] Gephyrin Human Mouse IgG2a 188193 Anti-Cav1.2... channel Rat Mouse IgG2a 199397 Anti-Gephyrin [L106/23R] Gephyrin Human Mouse IgG2a 199398 Anti-Gamma-...] QKI-7b Human Mouse IgG2a 206541 Anti-PhyH/PAHX [N210/5R] PhyH/PAHX Human Mouse IgG2a 206542 Anti-Lhx6.1... -
AAV Molecular Tools
TypeCollection..., Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments...Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators ...Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP for labeling of axon terminals. 1 Zeng ...Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression ...expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals. 1 Luo 60658 pAAV-EF1α-...of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato 8 Scanziani Tools for Cell...Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6 Zhang Don’t See What You... -
Cre-lox system
TypeCollection...131781 pCS2+PhyBΔCreC CreLite - Red light-inducible Cre; C terminus Cre fused to PhyBdelta CMV Mammalian...131782 pME-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit...131785 pAAV-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector Cbh...131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral...Chicken cardiac troponin T AAV Pu 134308 pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre Cre-ERT2 - Tamoxifen ...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre... -
Retrograde AAV viral preps
TypeCollection...55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Deisseroth...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Deisseroth...AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting ...-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium...AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting ... -
Bacterial Expression Systems
TypeCollection...from the culture and purified through affinity chromatography. To facilitate the purification process, proteins...68940 pRMC2 Pxyl/TetO Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267 pBAD33.1 pBAD Arabinose...glutamicum Timothy Lu 36252 pRibo EcoHind Riboswitch Teophylline Escherichia coli , Mycobacterium smegmatis Carolyn...Jessica Seeliger 36251 pRibo BsaHind Riboswitch Teophylline Escherichia coli , Mycobacterium smegmatis Carolyn... -
Microbiology Resources
TypeCollection... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains... Rickettsia sp. Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus... -
CRISPR History and Development for Genome Engineering
TypeCollection...Cas9 orthologs have also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and ...immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun . 439(1):132-6. PMID: 23942116 Gilbert...Zhang F. 2015. In vivo genome editing using Staphylococcus aureus Cas9. Nature . 520(7546):186-91. PMID... -
Neurodegeneration Plasmid Collection
TypeCollection...72286 pCI SMN1 SMN1 CMV Spinal muscular atrophy Elliot Androphy 72873 PMXS-SLC1A3 SLC1A3 Episodic ataxia...Riggs 37057 GFP-SMN SMN1 GFP CMV Spinal muscular atrophy Greg Matera 38005 pET-21a(+)/MBP-His-PINK1 PINK1...Schmitt-Ulms 61946 4QQ6 SMN1 His T7 Spinal muscular atrophy Cheryl Arrowsmith 65106 pcDNA3-ADAM10-HA ADAM10...SMN1 myc HIS SMN1 His, Myc CMV Spinal muscular atrophy Gideon Dreyfuss 71875 pET-Sac-Abeta(M1-42) APP ...156022 SMN1-1 SMN1 V5-MCP EF1a Spinal muscular atrophy Eugene Yeo 156073 TARDBP TARDBP V5-MCP EF1a ALS...178154 COQ2_Halo_N_allele COQ2 Halo Multiple system atrophy Michael Ward 178155 DAO_Halo_C_allele DAO Halo ... Dystonia-parkinsonism, Infantile neuroaxonal dystrophy Michael Ward 178167 RNF216_Halo_C_allele RNF216... -
Ras Pathway
TypeCollection... removal of a phosphate group. Under normal physiological conditions GTPases play prominent roles in signaling...Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-95. PubMed PMID: 17428555... -
CRISPR Guide
TypeCollection... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus...Zhang, F. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. ...immunoprecipitation (enChIP) using CRISPR. Biochemical and Biophysical Research Communications . 439 (1), 132–136. PMID... -
Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids
TypeCollection...off-target effects Modified PAM specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered... -
Cancer Research Plasmids and Resources
TypeCollection...pathways are important to normal cell signaling and physiology, and, when dysregulated, can enable cancer cell... -
Fluorescent Protein Guide
TypeCollection...Biosensors Monitor small biomolecules or other physiological intracellular processes with genetically encoded... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection...for in vivo imaging are derived from bacterial phytochrome photoreceptors (BphPs). Spectrally distinct permanently... -
Structural Genomics Consortium Plasmids
TypeCollection...used for determining protein 3D structure and crystallography.... -
Chemogenetics Plasmids
TypeCollection...have been engineered to respond to specific physiologically inert small molecules. There are four types... -
Optogenetics Plasmid Collection
TypeCollection... light sensitive protein from cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins... -
Antibody Production
TypeCollection... columns. Formulation Buffer After affinity chromatography, antibodies undergo a buffer exchange using... -
Chemogenetics AAV Preps
TypeCollection...Gq-P2A-dTomato hM3D(Gq) - Activation dTomato (physically separate) none 1, 9, PHPeB Dimidschstein No available...