We narrowed to 2,171 results for: Xpa
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pEVOL-pBpF
Plasmid#31190PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.DepositorInsertM.j. p-benzoylphenylalanine RS (2 copies +tRNA)
ExpressionBacterialMutationY32G, E107P, D158T, I159SAvailable SinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pTECH-chAcK3RS(IPYE)
Plasmid#104069PurposeExpression plasmid encoding chAcK3RS(IPYE) and pylTDepositorInsertPlpp chAcK3RS(IPYE); PproK pyl
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD_FLAG-GFP(Y39TAG)-6His
Plasmid#223510PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
Tags6xHis and FLAGExpressionBacterialMutationY39TAGAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSerOTS-C1* (V70)
Plasmid#188537PurposePhosphoserine incorporation into recombinant proteinsDepositorInsertspSerRS
Ef-pSer
tRNApSer
ExpressionBacterialMutationmutations resulting from laboratory evolutionPromoterglnS*Available SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6/7FTrp)RS
Plasmid#207621PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbPylRS(IPYE)
Plasmid#104072PurposeExpression plasmid encoding MbPylRS(IPYE) and pylTDepositorInsertPlpp MbPylRS(IPYE); PproK pyl
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet3.0
Plasmid#174080PurposeTetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertTet3.0 tRNA synthetase and tRNA
ExpressionBacterialMutationL270G, Y271L, N311G, C313AAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mut1-RS
Plasmid#182881PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut1-RS
ExpressionBacterialAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY
Plasmid#64915PurposeEncodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertsPolyspecific aminoacyl-tRNA Synthetase
Amber Suppression tRNA
TagsNoneExpressionMammalianPromoterCMV and U6/H1Available SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAH-EcaYRS
Plasmid#153558PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in mammalian cellsDepositorInsertsEcaYRS
amber suppression tRNA (multiple copies)
ExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
AzoRS-4
Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
ExpressionBacterialAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MmPylRS(IPYE)
Plasmid#104073PurposeExpression plasmid encoding MmPylRS(IPYE) and pylTDepositorInsertPlpp MmPylRS(IPYE); PproK pyl
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbAcK3RS(IPYE)
Plasmid#104070PurposeExpression plasmid encoding MbAcK3RS(IPYE) and pylTDepositorInsertPlpp MbAcK3RS(IPYE); PproK pyl
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only
