We narrowed to 14,741 results for: nans

1. (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

   Plasmid

   #168097

   Purpose

   HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nGFP-(EAAAK)4-OGA(544-706)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNeae2

   Plasmid

   #168300

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

   Insert

   Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
   

   Use

   Tags

   6xHis, E-tag, and myc-tag

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriEx-HTNC

   Plasmid

   #13763

   Purpose

   For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

   Depositor

   Klaus Rajewsky

   Article

   Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

   Insert

   His-TAT-NLS-Cre
   

   Use

   Baculovirus

   Tags

   His Tag, NLS, and TAT

   Expression

   Bacterial

   Mutation

   Promoter

   p10 promoter

   Available since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE5max-SF3B1-K700E

   Plasmid

   #200317

   Purpose

   Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Inserts

   K700E-ng1+pg2
   PEmax-2A-MLH1dn
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-Laconic/pCMV-myc-nuc

   Plasmid

   #46307

   Purpose

   a nuclear-targeted, genetically-encoded sensor for lactate

   Depositor

   Luis Felipe Barros

   Article

   Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

   Insert

   NLS-Laconic
   

   Use

   Lactate biosensor

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-TRE-AFOS

   Plasmid

   #197079

   Purpose

   A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

   Depositor

   Elaine Fuchs

   Article

   Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

   Insert

   AFOS (Fos Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and T7

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKII-pAce-Kv2.1PR

   Plasmid

   #195529

   Purpose

   Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAce-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Ace-mNeon 78K, 81D, 92N, 178F; SY linker

   Promoter

   CaMKII

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLpA-DN.JNK1

   Plasmid

   #51942

   Purpose

   Adenoviral vector for dominant negative JNK1 expression

   Depositor

   Jeffery Molkentin

   Article

   Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

   Insert

   DN JNK1 (Mapk8 Mouse)
   

   Use

   Adenoviral

   Tags

   FLAG

   Expression

   Mutation

   dominant negative: the dual phosphorylation moti…

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. delCMV-mCherry-3x-p67phox-GBD

   Plasmid

   #214141

   Purpose

   Rac activity sensor

   Depositor

   Leif Dehmelt

   Article

   Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

   Insert

   3xp67phox-GBD (NCF2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Only the GTPase binding domain (GBD) is included

   Promoter

   delCMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-fDIO-pAce-Kv2.1PR

    Plasmid

    #195535

    Purpose

    Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAce-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 78K, 81D, 92N, 178F; SY linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. araBAD-BCD20-ClpP-BCD17-ClpX

    Plasmid

    #153301

    Purpose

    Expresses clpX and clpP in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Inserts

    clpX (clpX Escherichia coli)
    clpP (clpP Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-DIO-pAceR-Kv2.1PR

    Plasmid

    #195534

    Purpose

    Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR-Kv2.1 proximal restriction sequence
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. BLADE

    Plasmid

    #134912

    Purpose

    Empty backbone for cloning sgRNA sequence to be used in nanoblades system

    Depositor

    Théophile Ohlmann, Philippe Mangeot, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PR

    Plasmid

    #195532

    Purpose

    Cre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    Ace-mNeon2-Kv2.1 proximal restriction sequence
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 81S; SY linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-myc-EphB6

    Plasmid

    #200984

    Purpose

    Mammalian expression plasmid for myc-tagged EphB6

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB6 (EPHB6 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-DIO-VARNAM2-Kv2.1PR

    Plasmid

    #195533

    Purpose

    Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Syn-pAce-Kv2.1PR

    Plasmid

    #195523

    Purpose

    Green fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAce-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 78K, 81D, 92N, 178F; SY linker

    Promoter

    Synapsin

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Syn-Ace-mNeon2-Kv2.1PR

    Plasmid

    #195522

    Purpose

    Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    Ace-mNeon2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 81S; SY linker

    Promoter

    Synapsin

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA_B2AR-s-pep

    Plasmid

    #47438

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

20. BLADE-182

    Plasmid

    #134914

    Purpose

    sgRNA targeting GFP to be used in nanoblade system

    Depositor

    Théophile Ohlmann, Philippe Mangeot, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge