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pLenti4/V5-DEST-P85(K550_K562dup)
Plasmid#40224DepositorInsertP85(K550_K562dup) (PIK3R1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationcreated internal duplication of amino acids 550-5…PromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
mouse KGFR NEG D
Plasmid#19272DepositorInsertmKGFR (negative dominant) (Fgfr2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJK021
Plasmid#75060PurposeProduces Saccharomyces cerevisiae ADE2 (N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein)DepositorInsertphosphoribosylaminoimidazole carboxylase ADE2 (ADE2 Budding Yeast)
UseTagsExpressionBacterialMutationE. coli optimized codons in first 25 residuesPromoterlacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP TCS1
Plasmid#139161PurposeSB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.DepositorInsertCaffeine Synthase 1 [Camellia sinensis]
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CaDXMT
Plasmid#139165PurposeSB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes.DepositorInsert3,7-dimethylxanthine N-methyltransferase 1 [Coffea arabica]
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CsAncCS
Plasmid#139167PurposeSB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis ancestral caffeine synthase (CsAncCS); the other side contains RFP and puromycin resistance genes.DepositorInsertPredicted Camellia sinensis Ancestral Caffeine Synthase
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP PcAncCS2
Plasmid#139170PurposeSB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana ancestral Caffeine Synthase 2 (PcAncCS2); the other side contains RFP and puromycin resistance genes.DepositorInsertPredicted Paullinia cupana var sobilis Ancenstral Caffeine Synthase-2
UseTransposonTagsNoneExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CaMXMT
Plasmid#139172PurposeSB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.DepositorInsertmonomethylxanthine methyltransferase 1 [Coffea arabica]
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE
Plasmid#135391PurposeCre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.DepositorInsertTeLC-P2A-EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE-P48R
Plasmid#161816PurposeExpresses ABE-P48R in mammalian cellsDepositorInsertbpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Blast
Plasmid#83480PurposeMammalian expression of Cas9 and sgRNA scaffoldDepositorInsertBlasticidin S deaminase
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterEF-1αAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-sCAG-mBaoJin-oPRE
Plasmid#218684PurposeAAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInsertmBaoJin
UseAAVTagsExpressionMammalianMutationPromoterShort variant of the CAG promoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon DREADD Gi-mCherry
Plasmid#177672PurposeExpresses DREADD hM4D (Gi) in Cre and Flp expressing cellsDepositorHas ServiceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-LacI #1691
Plasmid#232636Purposelac repressor (LacI) which binds lacO sites; labeled with HaloTagDepositorInsertHaloTag-LacI
UseTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSExpressionMutationPromoterTRE, PGKAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#160389PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selectionDepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseCen/arsTagsExpressionYeastMutationPromoterEndogenous ATG8 promoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-HypaSpCas9-P2A-puro
Plasmid#106965Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64
Plasmid#203348PurposeTemplate for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertEnhanced Green Fluorescent Protein
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV-sig-mCherry-PDGFRTM-WPRE
Plasmid#216668PurposeExpresses membrane-bound mCherry in mammalian cellsDepositorInsertmCherry
UseTagsPDGFR Transmembrane DomainExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX 6P1 CDK2 (552)
Plasmid#61845Purposebacterial expression of GST CDK2 WTDepositorInsertCDK2 (CDK2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF274 SVAfw
Plasmid#178192PurposeThe SVA (SINE-VNTR-ALU) retroposon that causes human X-linked Dystonia Parkinsonism (XDP), within a BAC (pJazz) backbone.DepositorInsertSVA that inserts into the X-linked human TAF1 gene to cause X-linked dystonia Parkinsonism
UseLinear bac vectorTagsExpressionMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-EGFP
Plasmid#161776Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interestDepositorInsertH2B-EGFP from Addgene Plasmid #26790
UseLentiviral; BicistronicTagsEGFPExpressionMammalianMutationPromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSJL1
Plasmid#71261PurposeE. coli-Veillonella shuttle plasmidDepositorTypeEmpty backboneUseE. coli-veillonella shuttle plasmidTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_ mito_roGFP2-Orp1
Plasmid#155042PurposeFluorescent redox reporterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsmitochondrial targeting sequence and roGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H2B-iRFP720
Plasmid#128961PurposeLentiviral plasmid for expression of far red iRFP720 nuclear markerDepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterhPGKAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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CMV-eGFP-Z1
Plasmid#135049PurposeEnhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLX1.8NFATGFPpd2HS4
Plasmid#169096PurposeLentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activationDepositorInsertsNFAT enhancer (from IL-4 promoter)
Destabilized green fluorescent protein
UseLentiviralTagsExpressionMutationPromoterNFAT enhancer + synthetic TATA boxAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Banshee RunxDN mCherry
Plasmid#80158Purposeretroviral expression of Runx1DN and mCherryDepositorInsertRunx1 (Runx1 Mouse)
UseRetroviralTagsHA and IRES-mCherryExpressionMammalianMutationDN--truncated version of the Runx1 protein contai…PromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64
Plasmid#203350PurposeTemplate for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertNano Luciferase
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
M3-PAmcherry1
Plasmid#114189Purposestudying clustering of M3 receptors by superresolution microscopy (PALM)DepositorInsertMuscarin acetylcholine receptor 3 (CHRM3 Human)
UseTags3xHA and PAmCherryExpressionMammalianMutationPromoterSV40Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
POLG-pcDNA3.1
Plasmid#191487PurposeExpresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.DepositorInsertPOLG (POLG Human)
UseTagsV5 tag, 6X HisExpressionMammalianMutationPromoterCMV, T7Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-C1-centrin-1
Plasmid#73331PurposeLentiviral vector encoding centrin-1 with an N-terminal EGFP tagDepositorInsertcentrin-1 (CETN1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only
