Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,355 results for: bli

Showing: 4121 - 4160 of 9355 results

  1. pCG-scr

    Plasmid
    #229847
    Purpose
    Expresses wild-type Cas9 and scrambled gRNA.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA with scrambled sequence
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229847-plasmid-map-sequence-id-457515

    Enlarge

  2. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid
    #214682
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214682-plasmid-map-sequence-id-428816

    Enlarge

  3. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid
    #214683
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr2
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214683-plasmid-map-sequence-id-428804

    Enlarge

  4. pDual_dsCas9_Venus_hs_TP73

    Plasmid
    #214685
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214685-plasmid-map-sequence-id-428818

    Enlarge

  5. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid
    #214686
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214686-plasmid-map-sequence-id-428810

    Enlarge

  6. pDual_dsCas9_BFP_hs_TP73

    Plasmid
    #214687
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214687-plasmid-map-sequence-id-428806

    Enlarge

  7. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

    Enlarge

  8. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

    Enlarge

  9. pHR-TRE-miRFP703_PGK-Puro

    Plasmid
    #216667
    Purpose
    Expresses tTa-inducible miRFP703, with puromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    miRFP703
    PuroR
    Use
    Lentiviral
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216667-plasmid-map-sequence-id-432197

    Enlarge

  11. pAAV-phSyn-flex-SypHaloTag

    Plasmid
    #172280
    Purpose
    Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.
    Depositor
    Danny Winder
    Insert
    synaptophysin-HaloTag
    Use
    AAV and Cre/Lox
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    rat synapsin
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    172280-plasmid-map-sequence-id-337805

    Enlarge

  12. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Available Since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

    Enlarge

  13. pIND4-GFP-MmXYRS

    Plasmid
    #222745
    Purpose
    Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Steven Boxer
    Inserts
    sfGFP
    MmXYRS
    Mm Pyl tRNA
    Tags
    6xHis
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available Since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222745-plasmid-map-sequence-id-442565

    Enlarge

  14. H2B-Electra2

    Plasmid
    #218929
    Purpose
    Electra2 fused to human H2B protein for nuclear localization
    Depositor
    Amro Hamdoun
    Insert
    H2B-Electra2 (H2B Synthetic, Entacmaea quadricolor, Human)
    Use
    Synthetic Biology
    Tags
    Electra2 and H2B
    Expression
    Mammalian
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218929-plasmid-map-sequence-id-433882

    Enlarge

  15. LvPolyUb

    Plasmid
    #218982
    Purpose
    L.variegatus polyubiquitin-C promoter
    Depositor
    Amro Hamdoun
    Insert
    L.variegatus polyubiquitinC promoter
    Use
    Sea urchin
    Promoter
    L.variegatus polyubiquitinC promoter
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218982-plasmid-map-sequence-id-433881

    Enlarge

  16. pet28a+_Yjjg

    Plasmid
    #220228
    Purpose
    Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
    Tags
    Histag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    220228-plasmid-map-sequence-id-439539

    Enlarge

  17. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

    Enlarge

  18. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

    Enlarge

  19. 1167A

    Plasmid
    #218233
    Purpose
    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene
    Depositor
    Omar Akbari
    Insert
    putative metabolite transport protein
    Expression
    Insect
    Available Since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    218233-plasmid-map-sequence-id-433264

    Enlarge

  20. pET28-Flag-RBM24

    Plasmid
    #208621
    Purpose
    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208621-plasmid-map-sequence-id-433637

    Enlarge

  21. pET-29b(+)_notufA-EmEndoF2-CBD-His

    Plasmid
    #218197
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2
    Depositor
    Florence Pojer
    Insert
    EndoF2
    Tags
    CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-335
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218197-plasmid-map-sequence-id-431793

    Enlarge

  23. E3_pENTR_L3-L2_NI_G1333-DddA11-N

    Plasmid
    #191609
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191609-plasmid-map-sequence-id-379112

    Enlarge

  24. E3_pENTR_L3-L2_NN_G1333-DddA11-N

    Plasmid
    #191610
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191610-plasmid-map-sequence-id-379111

    Enlarge

  25. E1_pENTR_L1-L4_HD_G1333-DddA11-C

    Plasmid
    #191599
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191599-plasmid-map-sequence-id-379206

    Enlarge

  26. E1_pENTR_L1-L4_NG_G1333-DddA11-C

    Plasmid
    #191600
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191600-plasmid-map-sequence-id-379205

    Enlarge

  27. E1_pENTR_L1-L4_NI_G1333-DddA11-C

    Plasmid
    #191601
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191601-plasmid-map-sequence-id-379204

    Enlarge

  28. E1_pENTR_L1-L4_NN_G1333-DddA11-C

    Plasmid
    #191602
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191602-plasmid-map-sequence-id-379199

    Enlarge

  29. E3_pENTR_L3-L2_HD_G1333-DddA11-N

    Plasmid
    #191607
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191607-plasmid-map-sequence-id-379194

    Enlarge

  30. E3_pENTR_L3-L2_NG_G1333-DddA11-N

    Plasmid
    #191608
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191608-plasmid-map-sequence-id-379113

    Enlarge

  31. pT7CFE1_polio_VP12A

    Plasmid
    #212988
    Purpose
    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus type I Mahoney VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212988-plasmid-map-sequence-id-420361

    Enlarge

  32. pcDNA3-hFCER1G-VenusFlag

    Plasmid
    #211899
    Purpose
    Expresses human FCER1G in mammalian cells
    Depositor
    Haian Fu
    Insert
    FCER1G (FCER1G Human)
    Tags
    VenusFlag
    Expression
    Mammalian
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    211899-plasmid-map-sequence-id-419135

    Enlarge

  33. pNHA_OROV_NSs

    Plasmid
    #208934
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 69 bp at 5' end (including ATG) and …
    Promoter
    CMV
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208934-plasmid-map-sequence-id-418959

    Enlarge

  34. pPB-CAG-NPHP1

    Plasmid
    #209769
    Purpose
    Human NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    NPHP1 (NPHP1 Synthetic, Human)
    Use
    Piggybac transposon
    Tags
    HA tag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209769-plasmid-map-sequence-id-417213

    Enlarge

  35. pCR8GW-HA-Wnk

    Plasmid
    #203967
    Purpose
    HA-Wnk basic construct
    Depositor
    Andreas Jenny
    Insert
    Wnk (most similar to RO variant) (Wnk Fly)
    Use
    Unspecified
    Tags
    HA
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203967-plasmid-map-sequence-id-403297

    Enlarge

  36. pENTR3cDual-Fray

    Plasmid
    #203977
    Purpose
    Enrty clone for Gateway cloning
    Depositor
    Andreas Jenny
    Insert
    Frayed (fray Fly)
    Use
    Unspecified
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203977-plasmid-map-sequence-id-403301

    Enlarge

  38. PVALB mA_BCN14.1.1C9_LC_Mouse Ig Kappa

    Plasmid
    #182889
    Purpose
    Mammalian expression of PVALB mouse mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182889-plasmid-map-sequence-id-360569

    Enlarge

  39. PVALB mAb_BCN14.1.1C9_LC_Rat Ig Kappa

    Plasmid
    #182891
    Purpose
    Mammalian expression of PVALB rat mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182891-plasmid-map-sequence-id-360625

    Enlarge

  40. PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a

    Plasmid
    #182892
    Purpose
    Mammalian expression of PVALB mouse mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) heavy chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182892-plasmid-map-sequence-id-389490

    Enlarge

Showing: 4121 - 4160 of 9355 results