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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 10,975 results for: NSI;

Showing: 2061 - 2080 of 10975 results

  1. C103m

    Plasmid
    #121007
    Purpose
    MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121007-plasmid-map-sequence-id-236162

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  2. P35m

    Plasmid
    #120946
    Purpose
    MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).
    Depositor
    Richard Murray
    Insert
    pLbda-CasRQi
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120946-plasmid-map-sequence-id-235948

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  3. pCI_FLAG-GFP(Y39TAG)-6His

    Plasmid
    #223507
    Purpose
    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39
    Depositor
    Edward Lemke
    Insert
    GFP(Y39TAG)
    Use
    Tags
    6xHis and FLAG
    Expression
    Mammalian
    Mutation
    Y39TAG
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223507-plasmid-map-sequence-id-447435

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  11. pGL3_THADA_pos1toneg0

    Plasmid
    #232388
    Purpose
    THADA enhancer, one sensitizing element made into buffering Coordinator
    Depositor
    Joanna Wysocka
    Insert
    THADA (THADA Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    THADA enhancer, one sensitizing element made into…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232388-plasmid-map-sequence-id-462859

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  12. pGL3_THADA_alltoneg0

    Plasmid
    #232372
    Purpose
    THADA enhancer, all sensitizing elements made into buffering Coordinator
    Depositor
    Joanna Wysocka
    Insert
    THADA (THADA Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    THADA enhancer, all sensitizing elements made int…
    Promoter
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232372-plasmid-map-sequence-id-461186

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  13. KBB109

    Plasmid
    #185066
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after NsiI restriction site
    Depositor
    Ken Belanger
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup1 C-terminal region, truncated at NsiI restric…
    Promoter
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    185066-plasmid-map-sequence-id-367178

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  14. UCT1m

    Plasmid
    #121041
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UCT-part guide RNA (Stanley Qi sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121041-plasmid-map-sequence-id-245418

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  15. C114m

    Plasmid
    #121013
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9 with no bbsI or BsaI sites
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121013-plasmid-map-sequence-id-245424

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  17. UBC-4xmNG-dMCP

    Plasmid
    #231093
    Purpose
    Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).
    Depositor
    John Ngo
    Insert
    4xmNG-dMCP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231093-plasmid-map-sequence-id-458495

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  18. minCMV-1xmNG-dMCP

    Plasmid
    #231091
    Purpose
    Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).
    Depositor
    John Ngo
    Insert
    1xmNG-dMCP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231091-plasmid-map-sequence-id-458492

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  19. pCT02

    Plasmid
    #212152
    Purpose
    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
    Depositor
    Kang Zhou
    Insert
    FAST-PETase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S121E, D186H, R224Q, N233K, R280A
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212152-plasmid-map-sequence-id-424774

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Showing: 2061 - 2080 of 10975 results