We narrowed to 2,125 results for: PAM

1. LLPS1-iLID::EGFP::FTH1 (pBS1042)

   Plasmid

   #185289

   Purpose

   For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   iLID::eGFP::FTH1
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DRD5-DuET

   Plasmid

   #213231

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   DRD5 (DRD5 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ApoE3_mCh-SspB (pBS1144)

   Plasmid

   #185326

   Purpose

   For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE3
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FKBP-DCRD-YFP

   Plasmid

   #87450

   Purpose

   rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

   Depositor

   Xiaodong Liu

   Article

   Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

   Insert

   DCRD (distal C-terminal regulatory domain) (CACNA1F Human)
   

   Tags

   6xHis-T7, FKBP, and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ApoE2_mCh-SspB (pBS1142)

   Plasmid

   #185324

   Purpose

   For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE2
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CFP-PCRD-FRB

   Plasmid

   #87449

   Purpose

   rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

   Depositor

   Xiaodong Liu

   Article

   Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

   Insert

   PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
   

   Tags

   6xHis-T7, CFP, and FRB

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

   Plasmid

   #222876

   Purpose

   Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   FRB chain: V84R in GPA

   Promoter

   MSCV LTR

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LBR(R583Q)-FKBP-GFP

   Plasmid

   #222423

   Purpose

   To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   LBR(R583Q) (LBR Human)
   

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   R583Q

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LBR(N547D)-FKBP-GFP

   Plasmid

   #222422

   Purpose

   To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   LBR(N547D) (LBR Human)
   

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   N547D

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DRD3-DuET

    Plasmid

    #213229

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    DRD3 (DRD3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

    Plasmid

    #185299

    Purpose

    Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_3
    

    Expression

    Mammalian

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DHN_VrDHN1a_mCh-SspB (pBS1074)

    Plasmid

    #185298

    Purpose

    Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Expression

    Mammalian

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ApoE3_D125I_47-300_mCh-SspB (pBS1071)

    Plasmid

    #185294

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I_47-300
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ApoE3_D125I_mCh-SspB (pBS1145)

    Plasmid

    #185327

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ApoE4_mCh-SspB (pBS1143)

    Plasmid

    #185325

    Purpose

    For the mammalian expression of the human protein ApoE4 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE4
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)

    Plasmid

    #185309

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I_47-300
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pvLEA22mer_mutant_3_mCh-SspB (pBS1080)

    Plasmid

    #185303

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_3
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PvLEA4_repeats_k5_1_mCh-SspB (pBS1079)

    Plasmid

    #185302

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k5_1
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pvLEA22mer_mutant_10_mCh-SspB (pBS1078)

    Plasmid

    #185301

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_10
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAHS2_PARRC_98-130_mCh-SspB (pBS1077)

    Plasmid

    #185300

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2_PARRC_98-130
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge