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pCVD630

Plasmid

#66163

Purpose

cloning of ace gene

Depositor

James Kaper

Article

Trucksis et al Proc Natl Acad Sci U S A. 1993 Jun 1;90(

Insert

ace

Use

Cloning vector

Promoter

Unknown

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pND003

Plasmid

#200949

Purpose

Yeast surface display destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Use

Yeast surface display

Tags

AGA2, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pKM464 (deletions)

Plasmid

#108322

Purpose

Orbit integrating plasmid to delete target gene.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integration plasmid for creating knockouts …

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pKM468-EGFP

Plasmid

#108434

Purpose

ORBIT integrating plasmid for C-terminal tagging with EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-eGFP-4xGly-TEV-Flag-6xHis

Use

Orbit integrating plasmid

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pKM496 (deletions) Zeo

Plasmid

#109301

Purpose

ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK1126

Plasmid

#236187

Purpose

BsaI compatible gentamicin resistance cassette

Depositor

Jeffrey Barrick

Article

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

gentamicin resistance cassette

Expression

Bacterial

Promoter

CP25

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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psgRNA

Plasmid

#114005

Purpose

express sgRNA. ColE1, Kan

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

gRNA

Promoter

BBa_J23119

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pKM492-Myc-His

Plasmid

#109283

Purpose

ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Myc-His

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-GA

Plasmid

#191666

Purpose

ddGFP-A-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Tags

HA and ddGFP-A

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-B1

Plasmid

#191667

Purpose

ddFP-B1-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Tags

HA and ddFP-B1

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKM491-Flag-His

Plasmid

#109282

Purpose

ORBIT integrating plasmid for C-terminal tagging with FLAG-His tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Flag-His

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA Ago4

Plasmid

#10824

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 4 (AGO4 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-B3

Plasmid

#191668

Purpose

ddFP-B3-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Tags

HA and ddFP-B3

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKM493

Plasmid

#140191

Purpose

ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

TEV-Flag-Gly4-eGFP

Tags

Flag + eGFP

Expression

Bacterial

Promoter

PGroEL

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGGF007

Plasmid

#48847

Purpose

Provides a kanamycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::KanamycinR::tNOS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-jRCaMP1b

Plasmid

#233207

Purpose

Retroviral expression of jRCaMP1b calcium reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

jRCaMP1b

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IN

Plasmid

#60612

Purpose

Backbone for tet-inducible genes

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pNM3823

Plasmid

#154123

Purpose

mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::tetR-L-QFAD::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3755

Plasmid

#154116

Purpose

tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆pes-10::GFP-C1::act-4 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRN1120

Plasmid

#101750

Purpose

Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

Depositor

Rene Verwaal

Article

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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