We narrowed to 3,663 results for: golden gate

1. pPOM034_P7_3"Lys3

   Plasmid

   #216459

   Purpose

   3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   3''lys3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPOM038_P8b_5'Lys3

   Plasmid

   #216463

   Purpose

   5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   5'lys3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTBL2368 MTK PB puro backbone

   Plasmid

   #226673

   Purpose

   Empty MTK PB cargo backbone marked with puromycin resistance.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJB67

   Plasmid

   #226316

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with K to A CsoS2 MR1-6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K261A, K320A, K380A, K436A, K495A, K546A

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJB74

   Plasmid

   #226314

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1-5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A, Y359A, Y420A, Y475A, Y534A

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJB73

   Plasmid

   #226313

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A, Y359A, Y420A

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJB72

   Plasmid

   #226312

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJB45

   Plasmid

   #226304

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with C to S CsoS2 MR1-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C292S, C310S, C351S, C369S

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJB44

   Plasmid

   #226303

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with C to S MR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C292S, C310S

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJB43

    Plasmid

    #226302

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    WT CsoS2, frameshift site intact
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS416 CUP1pro-M-PRR-Htt97QP4-YFP

    Plasmid

    #224885

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.

    Depositor

    Paul Herman

    Insert

    M-PRR-Htt97QP4 (HTT Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    N-PRR

    Promoter

    CUP1

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJJB1426

    Plasmid

    #218622

    Purpose

    Express tNCS to the peroxisome for toxicity assay

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    tNCS
    

    Use

    Tags

    YFP-ePTS1

    Expression

    Yeast

    Mutation

    106-588

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJJB1462

    Plasmid

    #218623

    Purpose

    Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    YFP
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJJB1463

    Plasmid

    #218624

    Purpose

    Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    YFP
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJJB1734

    Plasmid

    #218627

    Purpose

    Express amorphadiene synthase (ADS)

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    ADS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJJB1535

    Plasmid

    #218613

    Purpose

    Express Pex5

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJJB1536

    Plasmid

    #218617

    Purpose

    Express Pex5 and Pex11

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJJB1376

    Plasmid

    #218618

    Purpose

    Express Pex5, Pex3, Pex19, and Pex8

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJJB1338

    Plasmid

    #218592

    Purpose

    Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex22
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Pex22(1-36)

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJJB207

    Plasmid

    #218596

    Purpose

    Express degron assay: Degradation tag-YFP-ePTS1

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    YFP
    

    Use

    Tags

    UbiY degradation tag and ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge