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Addgene

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We narrowed to 2,333 results for: Efs

Showing: 2081 - 2100 of 2333 results

  5. LV-AR

    Plasmid
    #213819
    Purpose
    Lenti-viral expression of human androgen receptor wild-type (WT).
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR (human androgen receptor) (AR Human)
    Use
    Lentiviral
    Tags
    Flag (N terminal on backbone)
    Expression
    Mammalian
    Mutation
    No, it is AR wild-type.
    Promoter
    EF-1a
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213819-plasmid-map-sequence-id-422263

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  11. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  12. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid
    #212196
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40
    Depositor
    Stacey Edwards
    Article
    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:
    Insert
    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    Use
    CRISPR and Lentiviral
    Tags
    HA, NLS, and blasticidin S deaminase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212196-plasmid-map-sequence-id-420116

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  16. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Article
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    115694-plasmid-map-sequence-id-221986

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  20. dCas9-p300 HAT

    Plasmid
    #179544
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179544-plasmid-map-sequence-id-354774

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Showing: 2081 - 2100 of 2333 results