We narrowed to 5,311 results for: cycs

1. pJ3 SHP2 CS

   Plasmid

   #8319

   Depositor

   Ben Neel

   Article

   Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C459S, mutation of essential catalytic cysteine

   Promoter

   Available since

   June 17, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-MARS-p85(iSH2)

   Plasmid

   #205236

   Purpose

   Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

   Depositor

   Jeremy Baskin

   Inserts

   PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
   p85(iSH2) (Pik3r1 Mouse)
   

   Use

   Tags

   Nuclear Export Sequence and mCherry

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1+_V5-ANKRD52

   Plasmid

   #92012

   Purpose

   Expresses V5-ANKRD52

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   ANKRD52 (ANKRD52 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT TO myc hSeparase

   Plasmid

   #59820

   Purpose

   Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC012 LwCas13a locus with nontargeting spacer

   Plasmid

   #91904

   Purpose

   LwCas13a locus into pACYC184 with nontargeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a with non-targeting spacer
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pJ3 SHP2 E76A

   Plasmid

   #8321

   Depositor

   Ben Neel

   Article

   Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E76A (activated mutant)

   Promoter

   Available since

   June 17, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTU387

   Plasmid

   #178981

   Purpose

   pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Insert

   CRISPR array from Candidatus Scalindua brodae
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. tetO-SMARCD3

   Plasmid

   #170701

   Purpose

   doxycycline-inducible overexpression of SMARCD3

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   SMARCD3 (SMARCD3 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS1068

   Plasmid

   #205962

   Purpose

   NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH proteins and CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU425

    Plasmid

    #178984

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT H585A C627A
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR4_CELF1

    Plasmid

    #106098

    Purpose

    Entry vector for CELF1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    CELF1 (CELF1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. tetO-SNAI2

    Plasmid

    #170702

    Purpose

    doxycycline-inducible overexpression of SNAI2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SNAI2 (SNAI2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEP301-pAAV-CMV-MCS3-pA

    Plasmid

    #113676

    Purpose

    CMV driven Multi Cloning Site-3

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    N/A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 FRT TO myc Separase C2029A

    Plasmid

    #59822

    Purpose

    Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC010 LshCas13a locus with nontargeting spacer

    Plasmid

    #91901

    Purpose

    LshCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with non-targeting spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGEX-SF3B1-NTerm

    Plasmid

    #97425

    Purpose

    Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

    Depositor

    Alex Minella

    Article

    Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

    Insert

    SF3B1-N-term (AA1-500)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Silent mutation at nt876, aa289 (AGG to AGA)

    Promoter

    tac

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1121 pcDNA3 GFP FKHR H215R

    Plasmid

    #9027

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H215R

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pBstSK+IRES-V5-IPpoI (mutant)

    Plasmid

    #206322

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag-mutated IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein (mutant version)

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

    Plasmid

    #136381

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NKP68 RFP (790)

    Plasmid

    #62003

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    NKP68 (TMUB1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    pgk

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only