We narrowed to 2,304 results for: ung

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

Plasmid

#226992

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

Depositor

Benjamin Kleinstiver

Publication

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 2b to create OPA1-del_exon5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

Plasmid

#226990

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

Depositor

Benjamin Kleinstiver

Publication

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 1b to create OPA1-del_exon5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

Plasmid

#226988

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

Depositor

Benjamin Kleinstiver

Publication

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A9 to create OPA1-R194G

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Publication

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_HD_160-223AA

Plasmid

#221477

Purpose

Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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plx304_CRKL-open-V5

Plasmid

#196023

Purpose

Expresses CRKL open, V5 tagged

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTmem132d#1/Cre

Plasmid

#193242

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tmem132d gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgTmem132d#1 (Tmem132d Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgTmem132d#2/Cre

Plasmid

#193243

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tmem132d gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgTmem132d#2 (Tmem132d Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSpen#1/Cre

Plasmid

#193239

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Spen gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSpen#1 (Spen Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSpen#2/Cre

Plasmid

#193240

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Spen gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSpen#2 (Spen Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSis#2/Cre

Plasmid

#193238

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Sis gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSis#2 (Sis Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgSis#1/Cre

Plasmid

#193237

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Sis gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSis#1 (Sis Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch3#2/Cre

Plasmid

#193228

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch3 gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch3#2 (Notch3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRos1#1/Cre

Plasmid

#193233

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Ros1 gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgRos1#1 (Ros1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRos1#2/Cre

Plasmid

#193234

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Ros1 gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgRos1#2 (Ros1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgFam135b#2/Cre

Plasmid

#193214

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Fam135b gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFam135b#1 (Fam135b Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgCol22a1#2/Cre

Plasmid

#193208

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Col22a1 gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCol22a1#2 (Col22a1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgAdgb#1/Cre

Plasmid

#193200

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Adgb gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgAdgb#1 (Adgb Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgMroh2b#2/Cre

Plasmid

#193220

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgMroh2b#2 (Heatr7b2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgAdgb#2/Cre

Plasmid

#193201

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Adgb gene

Depositor

Julien Sage

Publication

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgAdgb#2 (Adgb Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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