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We narrowed to 5,868 results for: nls

Showing: 4201 - 4240 of 5868 results
  1. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. LLP790_αGCN4-EZH2-FL-CO

    Plasmid
    #211784
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection
    Depositor
    Insert
    SnoopCatcher-KRAB
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pSK267

    Plasmid
    #129272
    Purpose
    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein
    Depositor
    Insert
    NLS-VP16-ZF 43-8-NES
    Use
    Tags
    6xHA
    Expression
    Yeast
    Mutation
    Promoter
    SUP35
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pYPQ230-RV

    Plasmid
    #188544
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing
    Depositor
    Insert
    LbCas12a-RV
    Use
    CRISPR; Gateway compatible lbcas12a-rv entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, R182V
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. MTK0_015

    Plasmid
    #123930
    Purpose
    Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
    Depositor
    Insert
    PB Destination - Insulator + tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. MTK0_017

    Plasmid
    #123932
    Purpose
    Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
    Depositor
    Insert
    BxBI attB tagBFP destination vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pX330-Flag-dHeFSpCas9

    Plasmid
    #92116
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Insert
    dead/inactive HeFSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    Cbh
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pTE3987

    Plasmid
    #137716
    Purpose
    Expresses dLbCas12a-RVRR-BE in mammalian cells.
    Depositor
    Insert
    dLbCas12a-RVRR-BE
    Use
    Tags
    6xHis, APOBEC-1, SV40 NLS, and UGI
    Expression
    Mammalian
    Mutation
    G532A, K538V, Y542R, K595R
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. JPF0522

    Plasmid
    #124013
    Purpose
    Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Insert
    PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126777
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Insert
    Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pYPQ230-D156R

    Plasmid
    #188542
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing
    Depositor
    Insert
    LbCas12a-D156R
    Use
    CRISPR; Gateway compatible lbcas12a-d156r entry c…
    Tags
    NLS
    Expression
    Plant
    Mutation
    D156R
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 4201 - 4240 of 5868 results