We narrowed to 7,344 results for: Lif

1. pDM1383

   Plasmid

   #207423

   Purpose

   Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

   Depositor

   Rob Kay

   Article

   Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

   Inserts

   HSPC300
   Raf1 (RAF1 Human)
   

   Use

   Unspecified

   Tags

   GFP and mCherry

   Expression

   Mutation

   aa 1–134 (RBD) only

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-ROR1-V5/HIS

   Plasmid

   #205607

   Purpose

   Expression of human ROR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   ROR1 receptor tyrosine kinase (ROR1 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-SARS2-Spike-D614G

   Plasmid

   #166850

   Purpose

   For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2 virus)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Sema3e(L)-Fc-His

   Plasmid

   #72145

   Purpose

   Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3e (Sema3e Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA pDEST40 Cas9-HMGB1

   Plasmid

   #183195

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   HMGB1
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CVS-N2c-nl.EGFP-jRGECO1a

   Plasmid

   #172386

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized EGFP + jRGECO1a
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol1-UAS:ChR2(H134R)-tdTomato

   Plasmid

   #124237

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   ChR2(H134R)-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. plKO.1-DNAJC5-ShRNA

   Plasmid

   #205729

   Purpose

   Knockdown of DNAJC5

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   shRNA targeting DNAJC5 (DNAJC5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 SPASTIN , residues 112-196

   Plasmid

   #180627

   Purpose

   Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 112-196

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sema6d.1-AP-His

    Plasmid

    #72042

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.1 (Sema6d Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. All-in-one split ADAR2(E488Q)

    Plasmid

    #170137

    Purpose

    AAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tom70NTD-ABHD16Acyto-mNG

    Plasmid

    #195159

    Purpose

    mammalian expression of outer mitochondrial membrane localized ABHD16A tagged with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    Tom70NTD (TOMM70 Human)
    

    Use

    Tags

    ABHD16A cytoplasmic domain and mNeonGreen

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sema4a-Fc-His

    Plasmid

    #72154

    Purpose

    Expresses the extracellular region of the Sema4A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4a (Sema4a Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

    Plasmid

    #125236

    Purpose

    Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
    

    Use

    AAV

    Tags

    Flag, V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-DNAJC5 (WT)-HaloTag

    Plasmid

    #205710

    Purpose

    Mammalian expression of DNAJC5 (WT)-HaloTag

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_BRD003 sgKEAP1-2

    Plasmid

    #74986

    Purpose

    sgKEAP1-2, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol1-UAS:Chronos-tdTomato

    Plasmid

    #124232

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    Chronos-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKrox24(2xD-E)DsRed

    Plasmid

    #200111

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-GST-hFIP200(1363-1370Mut)-MBP

    Plasmid

    #190038

    Purpose

    Expression of FIP200 mutant

    Depositor

    James Hurley

    Article

    Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    GST and MBP

    Expression

    Mammalian

    Mutation

    1363 RDKDLIES 1370 to GSSGGSSG

    Promoter

    CMV

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sema6d.1-Fc-His

    Plasmid

    #72168

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.1 (Sema6d Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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