We narrowed to 3,179 results for: UBC

1. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

   Plasmid

   #234882

   Purpose

   L1HS-silencing plasmid (CRISPRi gRNA1)

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   L1HS-silencing plasmid (CRISPRi gRNA1)
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL-c5e-Rubicon RH N821K, T911R, K916R

   Plasmid

   #216707

   Purpose

   Bacterial protein expression of Rubicon RH domain mutant

   Depositor

   James Hurley

   Article

   Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

   Insert

   Rubicon (RUBCN Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   N821K, T911R, K916R

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGG-GTS-TurboID-GFP

   Plasmid

   #209405

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   GTS-3XHA-TurboID
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGG-mTP-TurboID-GFP

   Plasmid

   #209404

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   mTP-3XHA-TurboID
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. iEscSnFR

   Plasmid

   #182807

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

   Insert

   iEscSnFR
   

   Use

   Tags

   6xHis, HA and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. iFluoxSnFR

   Plasmid

   #182808

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

   Insert

   iFluoxSnFR
   

   Use

   Tags

   6xHis, HA and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p8319 pDONR ATG-YAP1 WT-Stop

   Plasmid

   #184524

   Purpose

   Subcloning YAP1 WT

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

   Insert

   YAP1 WT (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. iSKetSnFR2

   Plasmid

   #140467

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.

   Depositor

   Henry Lester

   Article

   Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.

   Insert

   iSKetSnFR2
   

   Use

   Tags

   His Tag, HA Tag and Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. iSKetSnFR1

   Plasmid

   #140466

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR1, thus enabling optical subcellular pharmacokinetics for S-Ketamine.

   Depositor

   Henry Lester

   Article

   Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.

   Insert

   iSKetSnFR1
   

   Use

   Tags

   His Tag, HA Tag and Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIG1_UL136_19kDa_EE

    Plasmid

    #74951

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    Glu-Glu tag (EYMPME)

    Expression

    Mammalian

    Mutation

    delta 1-127

    Promoter

    CMV, lentiviral LTR

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIG1_UL136_23kDa_Myc

    Plasmid

    #74949

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    HA Tag (YPYDVPDYA)

    Expression

    Mammalian

    Mutation

    delta 1-99 M128A

    Promoter

    CMV, Lentiviral LTR

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAM5081

    Plasmid

    #85107

    Purpose

    Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    YFP-KaiC
    

    Use

    Tags

    YFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIG1_UL136_25kDa_Myc

    Plasmid

    #74954

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)

    Expression

    Mammalian

    Mutation

    delta 1-77, M100A M128A

    Promoter

    CMV, Lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUP95Q15ADSKGW (D7)

    Plasmid

    #74030

    Purpose

    lentiviral expression of Psd95-Q15A deltaSK-EGFP fusion

    Depositor

    Weifeng Xu, Robert Malenka, Oliver Schluter

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95 (DLG4 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q15A, delta SH3-GK (deletion of the fragment betw…

    Promoter

    hUbC

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCR4-*mRFP

    Plasmid

    #47118

    Purpose

    Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    *mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No start codon

    Promoter

    Lac

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. p198 Dync1i2.F (Ex1a)

    Plasmid

    #26448

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 24, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pUltra-MDH1-ME1

    Plasmid

    #184465

    Purpose

    Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Inserts

    Malate dehydrogenase 1 (MDH1 Human)
    Malic Enzyme 1 (ME1 Human)
    

    Use

    Lentiviral

    Tags

    fused to T2A

    Expression

    Mammalian

    Mutation

    deletion of stop codon

    Promoter

    UbC

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGK-DTAG-NTERM-GFP

    Plasmid

    #185775

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PGK-DTAG-CTERM-GFP

    Plasmid

    #185770

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304-IRES-Td

    Plasmid

    #176296

    Purpose

    pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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