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pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

Plasmid

#132684

Purpose

AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 2 (Pm20d2 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgSHMT2_2

Plasmid

#106310

Purpose

Express Cas9 and sgRNA targeting SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD8

Plasmid

#112820

Purpose

pSMF2 with 8 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD10

Plasmid

#112821

Purpose

pSMF2 with 10 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-OMM-diGlo

Plasmid

#251839

Purpose

Outer mitochondrial membrane targeted control glutamine biosensor

Depositor

Danielle Schmitt

Article

Scully et al bioRxiv [Preprint]. 2026 Jan 16:2026.01.

Insert

OMM-diGlo

Tags

DAKAP1 mitochondrial targeting motif

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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Dual_Reporter_SMN2_WT_Backbone

Plasmid

#247173

Purpose

Backbone reporter plasmid for exon skipping MPRA. The construct contains Citrine exon 1, SMN2 intron 6, SMN2 intron 7, and Citrine exon 2.

Depositor

Georg Seelig

Article

Koplik et al bioRxiv [Preprint]. 2025 Oct 13:2025.10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLaco1R1CI*_PLTetO1R1CI*

Plasmid

#250895

Purpose

Cassette 1: Mutated cI protein expressed under PL TetO1 promoter; Cassette 2: Mutated cI protein expressed under PL LacO1 promoter; part of BNeu AS10; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Mutated cI
Mutated cI

Use

Synthetic Biology

Mutation

cI* = Frame shifted cI protein

Promoter

PL LacO1 and PL TetO1

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLLacO1R3CIw_PLTetO1R2CIw

Plasmid

#250893

Purpose

Cassette 1: Wild type cI protein expressed under PL TetO1, regulated by RBS 'R2'; Cassette 2: Wild type cI protein expressed under PL LacO1 and regulated b RBS 'R3'; part of Bneu AS7; p15A origin

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Wild type cI
Wild type cI

Use

Synthetic Biology

Promoter

PL LacO1 and PL TetO1

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Hygro-EF1A>{H2B-RFP}

Plasmid

#249059

Purpose

Nuclear RFP labeling

Depositor

Ori Bar-Nur

Article

Gjonlleshaj et al Cell Rep Methods. 2026 Feb 23;6(2):10129

Insert

H2B

Use

Lentiviral

Tags

mRFP1

Promoter

EF1a

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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ARL13B-(Y86C)-GFP

Plasmid

#246840

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with Y86C mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Tyrosine 86 to Cysteine

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(G75Q)-GFP

Plasmid

#246837

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with G75Q mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13b (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed G 75 to Q

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-R-GFP

Plasmid

#246831

Purpose

Express Arl13B-GFP in Arl13B KO RPE1 cells (sg1#a), resistance to sgRNA

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13b-R-GFP (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(R79Q)-GFP

Plasmid

#246839

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with R79Q mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Arginine 79 to Glutamine

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(R200C)-GFP

Plasmid

#246841

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with R200C mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Arginine 200 to Cysteine

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(C8C9- SS)-GFP

Plasmid

#246842

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with C8C9-SS mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 8 and 9 to Serine

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(T35N)-GFP

Plasmid

#246838

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with T35N mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

change T 35 to N

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN12-Pct3-ElVadh(cHis6)-noAccs(DSM2243)

Plasmid

#233881

Purpose

Expresses Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh (Elen_0497 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN4-DDX6-sgDDX6-1 mut

Plasmid

#240009

Purpose

Expresses human DDX6 fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-sgDDX6-1 mut

Use

Lentiviral

Tags

3X Flag

Mutation

TTAAAAAAAGATAACATC

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN4-DDX6-CT

Plasmid

#240010

Purpose

Expresses human DDX6-CT fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-CT (303-483)

Use

Lentiviral

Tags

3X Flag

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN4-DDX6-NT

Plasmid

#240011

Purpose

Expresses human DDX6-NT fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-NT (1-302)

Use

Lentiviral

Tags

3X Flag

Mutation

TTAAAAAAAGATAACATC

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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