Addgene

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Launchpad (AVA2590)

Plasmid

#85442

Purpose

Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

Depositor

Joan Steitz

Article

Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

Insert

FRT-flanked EF1alfa-GFP PGK-Puromycin cassette

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only
pPB-EOS-GFP-IP

Plasmid

#60439

Purpose

PiggyBac for EOS-GFP

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

EOS

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGGF012

Plasmid

#48849

Purpose

Provides a sulfadiazine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::SulfadiazineR::t35S

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

internal BsaI recognition sites in SulfR removed …

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe puro MTH1

Plasmid

#21295

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pQIq_MRGS_HIS8_(27F3)_FLAG

Plasmid

#199615

Purpose

Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27F3

Use

Tags

8xHis and FLAG

Expression

Bacterial

Mutation

Promoter

T5

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPL5628_TEF1*-Cre_MET15

Plasmid

#60933

Purpose

Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Use

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Mutation

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pY3

Plasmid

#218116

Purpose

Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCCM935

Plasmid

#58202

Purpose

Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

unc-22 sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
LI F-G Spec

Plasmid

#104521

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Spectinomycin resistance gene

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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VZV ORF47

Plasmid

#26698

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

ORF47 (ORF47 Varicella Zoster Virus (HHV-3))

Use

Tags

HA

Expression

Mammalian

Mutation

A 3B3 epitope tag (DQRQYGDVFKGD) from the VZV gB …

Promoter

Available since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only
pDM1079

Plasmid

#108979

Purpose

Knockout cassette Blasticidin

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette Blasticidin

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Tags

Expression

Mutation

Promoter

coaA

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe hygro MTH1

Plasmid

#21296

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
GatA_pET29b

Plasmid

#215406

Purpose

Expression construct for GatA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

GatA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pQIq_MRGS_HIS8_(27G2)_FLAG

Plasmid

#199616

Purpose

Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27G2

Use

Tags

8xHis and FLAG

Expression

Bacterial

Mutation

Promoter

T5

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMyc0ElbLuc

Plasmid

#53242

Purpose

reporter plasmid without myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMycOElbLuc (MYC Human)

Use

Tags

Expression

Mutation

Promoter

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pET41-GST-KaiC

Plasmid

#68051

Purpose

Strain: AM5117, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiC

Use

Tags

6xHis, GST, and S-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Chx10 3kb AP (CC#318)

Plasmid

#15205

Depositor

Connie Cepko

Article

Rowan et al Dev Biol. 2005 May 15. 281(2):240-55.

Insert

Chx10 3kb AP reporter (Vsx2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pTSTEP

Plasmid

#72334

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Article

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[Golic+] RRS-EGFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMEX174-hCALML3

Plasmid

#47600

Depositor

Emanuel Strehler

Article

Qian et al Protein Sci. 1998 Nov;7(11):2421-30.

Insert

CALM3 (CALML3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
LII destination

Plasmid

#104529

Purpose

LII backbone vector

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Type

Empty backbone

Use

Ralstonia solanacearum transformation vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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