We narrowed to 7,172 results for: 11

1. pMGE-T7

   Plasmid

   #153021

   Purpose

   Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

   Depositor

   Vito Valiante

   Article

   Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pEBG-Pak1 83-149

   Plasmid

   #12214

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Dominant negative Pak1: autoinhibitory domain, aa…

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-MCN-10His-mStayGold (E138D)

   Plasmid

   #211361

   Purpose

   Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   mStayGold
   

   Use

   Tags

   10xHis-tag

   Expression

   Bacterial

   Mutation

   E138D

   Promoter

   T7

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

   Plasmid

   #158963

   Purpose

   Cre-dependet Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Kesavan et al Development. 2020 Jun 8;147(11). pii: de

   Inserts

   hsp70l promoter
   floxed Non-FP cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0078 RESCUE

   Plasmid

   #130661

   Purpose

   pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b-ADAR2dd (RESCUE)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HA-rat Nrxn2beta AS4(-)

   Plasmid

   #58268

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn2beta AS4(-) (Nrxn2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSC168

   Plasmid

   #216350

   Purpose

   xhNup35-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

   Plasmid

   #63166

   Purpose

   GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-HA"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSP1E2

    Plasmid

    #20063

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E2 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+ Dkk1-flag

    Plasmid

    #16690

    Depositor

    Xi He

    Article

    Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

    Insert

    Dkk1 (DKK1 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSP1E1

    Plasmid

    #20062

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeat of hel…

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDG04163

    Plasmid

    #216373

    Purpose

    xhNup214-Nb1t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup214-Nb1t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-scSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEnt L1L3 EF1a-tTA-2

    Plasmid

    #26803

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Elongation Factor 1 alpha promoter and tTA transgene
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. SIRT1 Flag

    Plasmid

    #13812

    Purpose

    Mammalian expression of human SIRT1 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    N-terminus truncated

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTP728

    Plasmid

    #216374

    Purpose

    xNup358-Nb1t (+3Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xNup358-Nb1t (+3Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMX-Brg1

    Plasmid

    #25855

    Depositor

    Hans Schöler

    Article

    Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

    Insert

    Brahma related gene 1 (Smarca4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-HA-rat Nrxn3beta AS4(-)

    Plasmid

    #58269

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn3beta AS4(-) (Nrxn3 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPUR-hU6-sgRNA-Sirius-8XMS2

    Plasmid

    #121942

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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