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Showing: 2121 - 2140 of 19449 results

  6. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183411-plasmid-map-sequence-id-361257

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  8. pMIGR AMPK KD

    Plasmid
    #27296
    Depositor
    Morris Birnbaum
    Insert
    AMP Acticated Protein Kinase (Prkaa2 Rat)
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Kinase Dead = Lys 45 to Arg
    Available since
    Sept. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    27296-plasmid-map-sequence-id-137644

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  11. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

    Plasmid
    #45574
    Purpose
    Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone
    Depositor
    Andrew Scharenberg
    Publication
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Insert
    co I-Sce I
    Use
    Lentiviral
    Tags
    HA, IRES BFP, and NLS
    Expression
    Mammalian
    Mutation
    co indicates codon optimization for mammalian exp…
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45574-plasmid-map-sequence-id-67191

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Showing: 2121 - 2140 of 19449 results