We narrowed to 3,421 results for: mpo

1. Lenti_CMV-Cas9-P2A-HygR

   Plasmid

   #164133

   Purpose

   Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9-EF-5Nanog_2

   Plasmid

   #174871

   Purpose

   CRISPR vector for generating Nanog STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Nanog (Nanog Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sACt-mCherry-NES

   Plasmid

   #138215

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   sACt-mCherry-NES (Adcy10 Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

   Plasmid

   #170177

   Purpose

   Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2s-ST-P2A-NLS-mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM-60-NusA-TEV-HsRCK_296-472_E

   Plasmid

   #146209

   Purpose

   Bacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsRck_296-472 (DDX6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Y951F-VEGFR2-mCherry

   Plasmid

   #139607

   Purpose

   Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FKBP-beta1(617-949)-GFP

   Plasmid

   #100732

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP1B1 (AP1B1 Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn-Aion-citrine

   Plasmid

   #192569

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Article

   Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

   Insert

   Aion
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRESneo-FLAG/HA-Ago2-Y529E

   Plasmid

   #215842

   Purpose

   Expression of AGO2-Y529E mutant

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ago2-Y529E

   Promoter

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. eSpCas9-EF-5Pou5f1

    Plasmid

    #174872

    Purpose

    CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IZU-LAMP1-mRuby3

    Plasmid

    #222586

    Purpose

    Stable expression of LAMP1-mRuby3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. (SUMO)5-(SIM)10

    Plasmid

    #126923

    Purpose

    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    internal BamHI site between (SUMO)5 and (SIM)10

    Promoter

    tac

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-MTS-WT-c-Src-FLAG

    Plasmid

    #44652

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Use

    Tags

    FLAG tag and mitochondria-targeting sequence (res…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. HT110_FCMV_QuasAr6b_citrine

    Plasmid

    #178823

    Purpose

    Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    QuasAr6b_citrine
    

    Use

    Lentiviral

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Y1214F-VEGFR2-mCherry

    Plasmid

    #139609

    Purpose

    Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSV272-PfMBP-HypDAE

    Plasmid

    #110296

    Purpose

    Expresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    t-4-Hydroxy-L-proline Dehydratase Activating Enzyme
    

    Use

    Tags

    His6 and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MO70-N-Flag-hCRACR2A-isoform-a

    Plasmid

    #79592

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

    Insert

    CRACR2A-isoform-a (CRACR2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

18. HT028_FCMV_CheRiff_EGFP

    Plasmid

    #178821

    Purpose

    Expresses a blue-light activated cationic channelrhodopsin CheRiff carrying an EGFP tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    CheRIff_EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. px459 sgAtg5

    Plasmid

    #175023

    Purpose

    CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    ATG5 (ATG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

    Plasmid

    #170175

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge