We narrowed to 6,085 results for: pcDNA 3.1

1. pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N

   Plasmid

   #193999

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181-D174N

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181-D174N
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3

   Plasmid

   #73208

   Purpose

   Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

   Insert

   COXIV-COX8-dL5-2XG4S-mCer3 (COX8A Synthetic, Human)
   

   Tags

   The FAP and mCerulean3 are fused with 2 copies of…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available Since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

   Plasmid

   #113949

   Purpose

   mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   residues 21-852

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. (112) pcDNA3.1-GFP-Flag-Nup62-EPEA

   Plasmid

   #168101

   Purpose

   GFP and Flag tagged Nup62 with EPEA nanobody tag

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   GFP-Flag-Nup62-EPEA
   

   Tags

   EPEA, Flag, and GFP

   Expression

   Mammalian

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

   Plasmid

   #164013

   Purpose

   Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R464E

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

   Plasmid

   #163991

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.1(-) mouse C/EBP beta (LIP)

   Plasmid

   #12561

   Depositor

   Peter Johnson

   Insert

   CCAAT/Enhancer-binding Protein beta LIP (Cebpb Mouse)
   

   Expression

   Mammalian

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

   Plasmid

   #49060

   Purpose

   Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Tags

   3x Flag and YFP (chloride-sensitive)

   Expression

   Mammalian

   Mutation

   Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…

   Promoter

   CMV

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 His-GFP-EWSR1-myc-His

   Plasmid

   #46385

   Purpose

   expresses EGFP tagged EWSR1 in mammalian cells

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   EGFP, His, and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

10. (383) pcDNA3.1-HA-nBC2-OGA(544-706)

    Plasmid

    #168100

    Purpose

    HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nBC2-OGA(544-706)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1-Flag-H2A K13-15-118-119R

    Plasmid

    #63568

    Purpose

    Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 13, 15, 118, and 119 are now Arginine

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127321

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid

    #73205

    Purpose

    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    NLS-myc-dL5-2XG4S-mCer3 (MYC Synthetic, Human)
    

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

    Plasmid

    #168192

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-LaG6-(EAAAK)4-OGT(4)
    

    Tags

    HA-Tag

    Expression

    Mammalian

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. (318) pcDNA3.1-myc-OGA(1-400)D174N

    Plasmid

    #168096

    Purpose

    Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    myc-OGA(1-400, D174N)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D174N catalytically inactive OGA

    Promoter

    T7/CMV

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-TAT-1-101-FLAG basic mutant

    Plasmid

    #35985

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV1, 101 aa; R to A mutations at aa 56,…

    Available Since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

    Plasmid

    #125796

    Purpose

    for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    CD40L (lose of function mutant)-mgSrtA
    

    Expression

    Mammalian

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

    Plasmid

    #163988

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    M1457A

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

    Plasmid

    #166804

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S1213A

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBD

    Plasmid

    #102606

    Purpose

    Mammalian expression of mutant cGAS with deletion of DNA binding domain fused to PSTCD tag

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Expression

    Mammalian

    Mutation

    delta K173-I220 and delta H390-C405

    Promoter

    CMV

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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