Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 3,167 results for: tef

Clear keywords
Showing: 2121 - 2140 of 3167 results

  1. pCRISPEY-GAL-Hyg-ADE2

    Plasmid
    #232104
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232104-plasmid-map-sequence-id-462310

    Enlarge

  2. pCas3-CDA1

    Plasmid
    #229534
    Purpose
    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Publication
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229534-plasmid-map-sequence-id-458891

    Enlarge

  9. pAG36-NPL3 S411A-3xHA

    Plasmid
    #262528
    Purpose
    Expresses NPL3 S411A with C-terminal 3xHA tag under its native promoter
    Depositor
    Michael Downey
    Insert
    Npl3 (NPL3 Budding Yeast)
    Tags
    3xHA
    Expression
    Yeast
    Mutation
    Serine 411 has been changed to alanine. Sequence …
    Promoter
    Native
    Available since
    Oct. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    262528-plasmid-map-sequence-id-524272

    Enlarge

  10. pCY5.1kh

    Plasmid
    #160297
    Purpose
    Yeast CRISPR plasmid targeting the kanMX and hphMX cassettes
    Depositor
    Amir Aharoni
    Publication
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    160297-plasmid-map-sequence-id-484567

    Enlarge

  11. pCY5.1nh

    Plasmid
    #160299
    Purpose
    Yeast CRISPR plasmid targeting the natMX and hphMX cassettes
    Depositor
    Amir Aharoni
    Publication
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160299-plasmid-map-sequence-id-464600

    Enlarge

  12. pdnCas3-CDA1

    Plasmid
    #229535
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Publication
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229535-plasmid-map-sequence-id-459013

    Enlarge

  13. pCas3-APOBEC1

    Plasmid
    #229536
    Purpose
    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Publication
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229536-plasmid-map-sequence-id-458911

    Enlarge

  16. pTF257 (pFA6a-TEV-6xGly-His3MX)

    Plasmid
    #44068
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, (XhoI-KpnI-XbaI)-containing spacer,…
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44068-plasmid-map-sequence-id-61660

    Enlarge

  17. pTF258 (pFA6a-PSc-6xGly-His3MX)

    Plasmid
    #44062
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, (XhoI-KpnI-XbaI)-containing spacer,…
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44062-plasmid-map-sequence-id-61661

    Enlarge

  18. pTF261 (pFA6a-TEV-6xGly-HphMX)

    Plasmid
    #44080
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, (XhoI-KpnI-XbaI)-containing spacer,…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44080-plasmid-map-sequence-id-61664

    Enlarge

  19. pTF262 (pFA6a-PSc-6xGly-HphMX)

    Plasmid
    #44074
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, (XhoI-KpnI-XbaI)-containing spacer,…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44074-plasmid-map-sequence-id-61665

    Enlarge

  20. pTF259 (pFA6a-TEV-6xGly-KanMX)

    Plasmid
    #44092
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, (XhoI-KpnI-XbaI)-containing spacer,…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44092-plasmid-map-sequence-id-61662

    Enlarge

Showing: 2121 - 2140 of 3167 results