We narrowed to 5,443 results for: SPI

1. pCAGEN-WPRE-bGH-DEST (JDW 1309)

   Plasmid

   #229815

   Purpose

   A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-3x-FLAG-Ubiquitin (JDW 1098)

   Plasmid

   #224513

   Purpose

   A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   3x-FLAG-Ubiquitin
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

   Plasmid

   #203844

   Purpose

   Flp-dependent Cre and mRuby3 expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   mRuby3-2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

   Plasmid

   #203842

   Purpose

   Flp-dependent Cre and EGFP expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   EGFP-2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-BLU-VIPR

   Plasmid

   #220497

   Purpose

   Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

   Depositor

   Alexander Espinosa

   Article

   Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLU-VIPR

   Plasmid

   #220498

   Purpose

   Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

   Depositor

   Alexander Espinosa

   Article

   Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-f:PGC1

   Plasmid

   #1026

   Depositor

   Bruce Spiegelman

   Article

   Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

   Insert

   PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIP-cathepsinD-YMH

   Plasmid

   #182644

   Purpose

   Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   cathepsinD
   

   Use

   Retroviral

   Tags

   His6-tag, TS site from proCCK, and myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

   Plasmid

   #203838

   Purpose

   Flp-dependent Cre and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   mScarlet-KASH-2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-mScarlet3-Giantin (JDW 1356)

    Plasmid

    #224502

    Purpose

    Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    mScarlet3-Giantin
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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