We narrowed to 6,038 results for: WUS;

1. LV-hNG2-GFP

   Plasmid

   #183910

   Purpose

   Expression of GFP under the human NG2 promoter

   Depositor

   Chun-Li Zhang

   Article

   Tai et al Cell Stem Cell. 2021 May 6;28(5):923-937

   Insert

   GFP (GFAP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEVOL-AckRS

   Plasmid

   #137976

   Purpose

   sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

   Depositor

   Wenshe Liu

   Article

   Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

   Insert

   AckRS and pylTcua
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-CMV-Galpha-q ONE-GO

   Plasmid

   #189738

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-q ONE-GO
   GNAQ (Gnaq Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-mito-GEM-GECO1

   Plasmid

   #32461

   Purpose

   Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO
   

   Use

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   CMV

   Available since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6His-MBP-TEV-huAsCpf1

   Plasmid

   #90095

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huAsCpf1
   

   Use

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF_PE2

   Plasmid

   #199267

   Purpose

   Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   PE2-P2A-mp53DD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-FAB(mito-LD)

   Plasmid

   #214421

   Purpose

   Detection of mito-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and RspAN-3XHA-V5

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15-ZmPYK

   Plasmid

   #220230

   Purpose

   Inducible expression of Zymomonas mobilis pyruvate kinase

   Depositor

   Liskin Swint-Kruse, Aron Fenton

   Article

   Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

   Insert

   Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV7.1_3xFLAG-LATS2

   Plasmid

   #172987

   Purpose

   constitutive expression of FLAG-tagged LATS2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-LATS2 (LATS2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti_SaCRISPR_GFP

    Plasmid

    #118636

    Purpose

    To clone sgRNA for SaCas9 (SauCas9)

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_MYC deltaCTD_P2A_Hygro_Barcode

    Plasmid

    #120511

    Purpose

    Barcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaCTD (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of carboxy-terminal domain

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_MYC deltaNTD_P2A_Hygro_Barcode

    Plasmid

    #120510

    Purpose

    Barcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaNTD (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of amino-terminal domain

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_MYC deltaMBI_P2A_Hygro_Barcode

    Plasmid

    #120504

    Purpose

    Barcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaMBI (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of MYC Box I domain

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a_MYC deltab_P2A_Hygro_Barcode

    Plasmid

    #120507

    Purpose

    Barcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltab (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of basic motif

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_MYC deltaMBII_P2A_Hygro_Barcode

    Plasmid

    #120505

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    c-MYC deltaMBII (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of MYC Box II domain

    Promoter

    EF1a

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_MYC deltaLZ_P2A_Hygro_Barcode

    Plasmid

    #120509

    Purpose

    Barcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaLZ (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of leucine zipper motif

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a_MYC deltaHLH_P2A_Hygro_Barcode

    Plasmid

    #120508

    Purpose

    Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaHLH (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of helix-loop-helix motif

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-Puro-hGSDME

    Plasmid

    #154876

    Purpose

    Expresses hGSDME in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pY108 (lenti-AsCpf1)

    Plasmid

    #84739

    Purpose

    Lenti virus delivery of AsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. AYBEv3

    Plasmid

    #193967

    Purpose

    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G163R, N169S, S198A, K202A, G203A, S206A, K210A

    Promoter

    hU6, EF1a, CBh

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGLOW31

    Plasmid

    #172698

    Purpose

    C. elegans mScarlet co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mScarlet
    

    Use

    Tags

    mScarlet

    Expression

    Worm

    Mutation

    Promoter

    myo-3

    Available since

    Aug. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. EF1a_KLF15_P2A_Hygro_Barcode

    Plasmid

    #120501

    Purpose

    Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF15 (KLF15 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-Vamp3-pHluorin

    Plasmid

    #190152

    Purpose

    Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp3 (Vamp3 Rat)
    

    Use

    AAV

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. Murine E-cadherin mCherry

    Plasmid

    #71366

    Purpose

    mammalian expression of murine E-caderin with mCherry fusion

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    E-cadherin (Cdh1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENN491_TALEcharm_MmT8x

    Plasmid

    #220836

    Purpose

    TALEcharm Kv2 expression in mammalian cells; targets Prnp

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    TALEcharm
    

    Use

    Lentiviral and TALEN

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EF1a

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lenti-mApple-6xHp

    Plasmid

    #217376

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    mApple-6x Hp NC
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mApple-6xHp

    Plasmid

    #214410

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    Spastin aa 43-92 (SPAST Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RfxCas13d-backbone

    Plasmid

    #165078

    Purpose

    Expression of RfxCas13d sgRNAs from a U6 promoter

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    Scaffold gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV-NLS-R-GECO

    Plasmid

    #32462

    Purpose

    Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Tags

    NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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