We narrowed to 4,147 results for: Alk

1. pT7-EGFP-C1-HsGIGYF2_1-718-del280-310_AI

   Plasmid

   #148949

   Purpose

   Mammalian Expression of HsGIGYF2_1-718-del280-310

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

   Insert

   HsGIGYF2_1-718-del280-310 (GIGYF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation and one deletion of Q1211 com…

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-EGFP-C1-HseIF4G1-L640AV646A_AD

   Plasmid

   #148520

   Purpose

   Mammalian Expression of HseIF4G1-L640AV646A

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   HseIF4G1-L640AV646A (EIF4G1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation M432V (natural variant is…

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD

   Plasmid

   #148521

   Purpose

   Mammalian Expression of HseIF4G1-LIVVtoAAAA

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   HseIF4G1-LIVVtoAAAA (EIF4G1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation M432V (natural variant is…

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD

   Plasmid

   #148522

   Purpose

   Mammalian Expression of HseIF4G1-Y619AL624AL625A

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   HseIF4G1-Y619AL624AL625A (EIF4G1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation M432V (natural variant is…

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACE-Truncated-Kir3.2-StreptagII

   Plasmid

   #177263

   Purpose

   Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

   Insert

   G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
   

   Use

   Tags

   StreptagII

   Expression

   Insect

   Mutation

   Residues 49-378

   Promoter

   polyhedrin promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LCV2_mClover3_PSMD1_sgRNA_1

   Plasmid

   #155102

   Purpose

   lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   PSMD1_sgRNA_1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LCV2_mClover3_AAVS1_sgRNA_4

   Plasmid

   #155101

   Purpose

   lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCKO_AAVS1_sgRNA_3

   Plasmid

   #155086

   Purpose

   lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LCV2_mCherry_AAVS1_sgRNA_4

   Plasmid

   #155106

   Purpose

   lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLCKO_AAVS1_sgRNA_4

    Plasmid

    #155087

    Purpose

    lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LCV2_AAVS1_sgRNA_4

    Plasmid

    #155090

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-2TKN-bp1

    Plasmid

    #133428

    Purpose

    Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    SREBF1 (SREBF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    SREBP1a amino acids 1-50 fused to GST

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-Lrrc40-trunc-IRES-BFP

    Plasmid

    #117268

    Purpose

    Retroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFP

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    Lrrc40 (Lrrc40 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The insert is truncated - deletion of bp 334-407 …

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA tagged SphI ELL2 M133, 138, 186ILeu

    Plasmid

    #127277

    Purpose

    Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains HA tag near C-terminus

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 with Met 133, 138, 186 to Ileu (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met 133, 138, 186 to Ileu; HA inserted at SphI af…

    Promoter

    pEF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe 3XFLAG-T34A-GATA6-3XAU1 puro

    Plasmid

    #72609

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutation

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed threonine34 to alanine

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pBabe 3XFLAG-S33A-GATA6-3XAU1 puro

    Plasmid

    #72610

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine33 to alanine

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pEN_TT 3XFLAG-S37A-GATA6-3XAU1

    Plasmid

    #72613

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Mutation

    Changed serine37 to alanine

    Promoter

    TRE tight promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

    Plasmid

    #72611

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine33, threonine34 and serine37 to ala…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pMIR-Report-RASA1-3'UTR (mut - miR-206 site)

    Plasmid

    #62576

    Purpose

    Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    RASA1 (RASA1 Human)
    

    Use

    Luciferase

    Tags

    None

    Expression

    Mutation

    miR-206 binding site mutated (ACATTCCA --> AAC…

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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