We narrowed to 29,109 results for: PLE;

1. pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro

   Plasmid

   #120559

   Purpose

   Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   HRAS (HRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

   Plasmid

   #120563

   Purpose

   Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

   Plasmid

   #107407

   Purpose

   Lentiviral expression of gRNA resistant PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   sg resistant PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   sgRNA resistant

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV TRE3G Neo GFP-Progerin C661S

   Plasmid

   #118711

   Purpose

   Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

   Depositor

   Tom Misteli

   Article

   Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

   Insert

   GFP-Progerin C661S (LMNA Human)
   

   Use

   Lentiviral; Tet-on inducible

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C661S farnesylation mutant

   Promoter

   CMV TRE3G (TET-ON)

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLENTI CMV GFP TREX1 R128A R174A BLAST

   Plasmid

   #164234

   Purpose

   lentiviral plasmid for GFP-TREX1 R128A R174A

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R124A R174A

   Promoter

   CMV

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

   Plasmid

   #122964

   Purpose

   Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Insert

   human Chibby1 (CBY1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   FoxJ1 and human FoxJ1

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

   Plasmid

   #83107

   Purpose

   3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide

   Depositor

   Andrew Brooks

   Inserts

   c-Myc (MYC Human)
   CDK4 (CDK4 Human)
   eGFP
   

   Use

   Lentiviral

   Tags

   2A self cleaving peptide

   Expression

   Mammalian

   Mutation

   R24C and p64 isoform

   Promoter

   hPGK and hPGK (single transcript)

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

   Plasmid

   #109428

   Purpose

   A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry T2A GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Inserted 43 base pairs into mCherry to create rep…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

   Plasmid

   #178574

   Purpose

   Lentiviral vector for CamKIIα::Opto-vTrap

   Depositor

   Won Do Heo

   Article

   Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

   Insert

   CRY2PHR-P2A-CIBN-mCherry-VAMP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

    Plasmid

    #178575

    Purpose

    Lentiviral vector for GFAP::Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CRY2PHR-P2A-CIBN-mCherry-VAMP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    GFAP

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP

    Plasmid

    #69048

    Purpose

    lentiviral expression of IKZF1 variant 1 with Q146H mutation and FLAG tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    splice variant 1, Q146H

    Promoter

    CAG

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47869

    Purpose

    All-in-one plasmid targeting tdTomato

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    tdTomato crRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGB3_alpha2: Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos-Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1679)

    Plasmid

    #160619

    Purpose

    Module for estradiol-inducible expression of the PhiC31 integrase gene and dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    ERLexABDGal4AD / PhiC31 / GRLacIBDGal4AD / RDF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Zyxin-Full Length (aa1-564)-EGFP

    Plasmid

    #187692

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

    Plasmid

    #191675

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

    Depositor

    Veerle Baekelandt

    Inserts

    ATP10B (ATP10B Human)
    TMEM30A (TMEM30A Human)
    

    Use

    Lentiviral

    Tags

    6X his and flag

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro

    Plasmid

    #118702

    Purpose

    lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    wt , SLC7A11 sh926R

    Promoter

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro

    Plasmid

    #120560

    Purpose

    Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    HRAS (HRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX304-GFP-IQSEC1 v2-E620K GEF dead mutant

    Plasmid

    #162020

    Purpose

    Expression of GEF dead IQSEC1 mutant

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EK

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47870

    Purpose

    All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    OCT4 crRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

    Plasmid

    #120564

    Purpose

    Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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