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pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3/S3

Plasmid

#124627

Purpose

Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 237 to 241 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_D290V_S285C

Plasmid

#98666

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C and D290V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

S285C D290V

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_D290V_S329C

Plasmid

#98668

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V and S329C

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

D290V S329C

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_LC_R191K_R201K_R216K_R254K

Plasmid

#104467

Purpose

express MBP hnRNPA2 LC with 4 R to K mutations

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

R191K R201K R216K R254K

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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AL62_pDonor.DMD.TS

Plasmid

#100287

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ETS1

Plasmid

#98621

Purpose

Gateway entry TOPO TA cloning vector for ETS1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETS1 (Ets1 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (PPP)

Plasmid

#62067

Purpose

Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A, P27A

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pCMV5b HA Irx5 deltaIrot

Plasmid

#24995

Depositor

Benoit Bruneau

Article

Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

Insert

Irx5 (Irx5 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of Iro box (aa 314-327), 19 aa upstream …

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5b HA Irx5 deltaC

Plasmid

#24996

Depositor

Benoit Bruneau

Article

Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

Insert

Irx5 (Irx5 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletes everything after Hox Domain (aa 112-177).…

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pLenti CMV Hygro dn-cHSF1

Plasmid

#134732

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Zeo dn-cHSF1

Plasmid

#134734

Purpose

Lentiviral expression vector for inducible expression of a constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of amino acids 186-201, 380−529

Promoter

CMV/TO

Availability

Academic Institutions and Nonprofits only

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pDest40 DHFR.dn-cHSF1

Plasmid

#134735

Purpose

Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G596C

Plasmid

#131712

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G596C

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G469S

Plasmid

#131716

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G469S

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-V600R

Plasmid

#131721

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600R

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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lenti MS2-P65-HSF1_Hygro

Plasmid

#61426

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-CBA-dt/PGK-CFTR-⌀

Plasmid

#163923

Purpose

Expresses human CFTR.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

CFTR (CFTR Human)

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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