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pDONR201-Eps8L2_544-715

Plasmid

#196532

Purpose

Entry vector for Gateway with Eps8L2_544-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_544-715 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_181-299

Plasmid

#196528

Purpose

Entry vector for Gateway with Eps8L2_181-299

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_181-299 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2208

Plasmid

#141969

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREB3L2 (CREB3L2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-H1DBD-H2

Plasmid

#200205

Purpose

Expression of HSF2 with HSF1 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-H1DBD-H2 (Hsf1, Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

HSF1 (1-399); HSF2 (366-1554)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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H2DBD-H1-Halo

Plasmid

#200193

Purpose

Expression of HSF1 with HSF2 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H2DBD-H1-Halo (Hsf1, Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

HSF2 (1-366); HSF1 (399-1576)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-Eps8L2_1-496

Plasmid

#196516

Purpose

Expression of GST-Eps8L2_1-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-496 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Mutation

Silent mutations for siRNA resistance

Promoter

lac promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_1-183

Plasmid

#196535

Purpose

Expression of GST-Eps8L2_1-183

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-183 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_181-299

Plasmid

#196536

Purpose

Expression of GST-Eps8L2_181-299

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_181-299 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_544-715

Plasmid

#196540

Purpose

Expression of GST-Eps8L2_544-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_544-715 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_529-715

Plasmid

#196533

Purpose

Entry vector for Gateway with Eps8L2_529-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_297-370

Plasmid

#196537

Purpose

Expression of GST-Eps8L2_297-370

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_297-370 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_367-496

Plasmid

#196538

Purpose

Expression of GST-Eps8L2_367-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_367-496 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_494-546

Plasmid

#196539

Purpose

Expression of GST-Eps8L2_494-546

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_494-546 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-hMIGA2 (170-593)

Plasmid

#192833

Purpose

Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX304-ARF5-T161A-mNeonGreen

Plasmid

#162026

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Mutation

T161A

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_D290V_S285C

Plasmid

#98666

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C and D290V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

S285C D290V

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_D290V_S329C

Plasmid

#98668

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V and S329C

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

D290V S329C

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_LC_R191K_R201K_R216K_R254K

Plasmid

#104467

Purpose

express MBP hnRNPA2 LC with 4 R to K mutations

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

R191K R201K R216K R254K

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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AL62_pDonor.DMD.TS

Plasmid

#100287

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ETS1

Plasmid

#98621

Purpose

Gateway entry TOPO TA cloning vector for ETS1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETS1 (Ets1 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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