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pMYT062

Plasmid

#180715

Purpose

Spacer cassette with ConL3 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

3/E Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT061

Plasmid

#180714

Purpose

Spacer cassette with ConL2 and ConR3 connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

2/3 Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT060

Plasmid

#180713

Purpose

Spacer cassette with ConL2 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

2/E Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT059

Plasmid

#180712

Purpose

Spacer cassette with ConL1 and ConR2 connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

1/2 Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT058

Plasmid

#180711

Purpose

Spacer cassette with ConL1 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

1/E Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT057

Plasmid

#180710

Purpose

Spacer cassette with ConLS and ConR1 connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

S/1 Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAW8_tdTomato

Plasmid

#110239

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein tdTomato

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_6XHis

Plasmid

#110236

Purpose

Cre/Lox C-terminal tagging construct encoding six histidine (His) residues

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_mCherry

Plasmid

#110238

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein mCherry

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_13Myc

Plasmid

#110232

Purpose

Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW32

Plasmid

#110225

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW33

Plasmid

#110226

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW34

Plasmid

#110227

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_1XFLAG

Plasmid

#110234

Purpose

Cre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_TAP

Plasmid

#110240

Purpose

Cre/Lox C-terminal tagging construct encoding the Twin Affinity Purification protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_3HA

Plasmid

#110233

Purpose

Cre/Lox C-terminal tagging construct encoding 3 copies of the influenza virus hemagglutunin (HA) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_yECitrine

Plasmid

#110231

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized yellow fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNX3a-HA3

Plasmid

#78359

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
kanMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDual_dsCas9_Puro_hs_NC_chr1

Plasmid

#214688

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

Depositor

Kevin Janes

Insert

dgRNA_Chr1

Use

CRISPR and Lentiviral

Mutation

Puromycin resistance cassette has silent mutation…

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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