We narrowed to 16,140 results for: igh

1. pC1-SypHer3s

   Plasmid

   #108118

   Purpose

   Ratiometric pH probe with enhanced brightness for mammalian cells expression.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s
   

   Use

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1(+)-NEMOc

   Plasmid

   #189933

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ORAI1-hBACCS2

   Plasmid

   #67626

   Purpose

   Mammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switch

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   ORAI1-hBACCS2
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1(+)-NEMOb

   Plasmid

   #189934

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-HcKCR1-EYFP

   Plasmid

   #182021

   Purpose

   Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

   Depositor

   Mingshan Xue

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR1
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-iLACCO1.2

   Plasmid

   #218823

   Purpose

   Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   iLACCO1.2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. V5-LOV-Turbo-NES

   Plasmid

   #199545

   Purpose

   expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   NES and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92392

   Purpose

   produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92390

    Purpose

    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. VPR-ps-dSpCas9

    Plasmid

    #102855

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    dSpCas9, pdDronpa1.2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS and VP64-p64-Rta transcription activator

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRY2PHR-mCherryN1

    Plasmid

    #26866

    Purpose

    Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2PHR (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-mNeptune2-N

    Plasmid

    #41645

    Purpose

    Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    mNeptune2-Dronpa145N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K145N on Dronpa (allows control of photochromic F…

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. TAL2451

    Plasmid

    #36840

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HMGA2-TALEN_Right (Hmga2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pAT253

    Plasmid

    #40943

    Depositor

    Angela Taddei

    Article

    Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

    Insert

    GFP-LacI**
    

    Use

    Tags

    GFP(S65T, V163A) and SV40 NLS

    Expression

    Yeast

    Mutation

    LacI**/LacI2G(4 mutations) (see comment below); l…

    Promoter

    His3 promoter fragment

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-iLACCO1.2

    Plasmid

    #218819

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNAHA

    Plasmid

    #44169

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Inserts

    hemagglutinin gene
    neuraminidase gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T55A

    Promoter

    CMV enhancer/human Ferritin light chain promoter …

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-BLU-VIPR

    Plasmid

    #220497

    Purpose

    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBLU-VIPR

    Plasmid

    #220498

    Purpose

    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDN77

    Plasmid

    #61347

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS2 and NES IkBa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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