We narrowed to 10,577 results for: kars;

1. 4xNLS-pMJ915v2-sfGFP

   Plasmid

   #88921

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-UBC-HA-gate-pGK-HYG

   Plasmid

   #107396

   Purpose

   Lentiviral gateway destination vector

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEFIRES-P-mTagBFP-KDEL

   Plasmid

   #87163

   Purpose

   Fluorescent marker of endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   mTagBFP-KDEL
   

   Use

   Tags

   Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1 alpha

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_083 Cas12i1

   Plasmid

   #171670

   Purpose

   For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TwinStrep-SUMO-NLS-BhCas12b_v4

   Plasmid

   #158615

   Purpose

   For protein expression of BhCas12b_v4

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   NLS-BhCas12b_v4
   

   Use

   Tags

   6xHIS-TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 BoMo N-1F

   Plasmid

   #203761

   Purpose

   For expression of N-flag tagged BoMo R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-ECFPEYFP

   Plasmid

   #24520

   Depositor

   Jehonathan Pinthus, Liusheng He

   Article

   He et al Cytometry A. 2003 Oct . 55(2):71-85.

   Insert

   ECFP, EYFP
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCHARGE3

   Plasmid

   #24658

   Depositor

   Tanya Parish

   Article

   Carroll et al PLoS One. 2010 . 5(3):e9823.

   Insert

   Turbo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6a:sgRNA(tyr)

   Plasmid

   #64250

   Purpose

   expresses sgRNA(tyr) under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   U6a:sgRNA (tyr)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. DHFR-myc

    Plasmid

    #20214

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBRα

    Plasmid

    #53731

    Purpose

    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    αNTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMito-TrxRFP1

    Plasmid

    #98997

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and mitochondrial localization sequence
    

    Use

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL059

    Plasmid

    #177210

    Purpose

    Bicistronic vector with GFP reporter

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MDH1-PGK-H2Kb2

    Plasmid

    #17852

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    H-2Kb (H2-K1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDsEndoNA2

    Plasmid

    #174728

    Purpose

    Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1

    Insert

    DsRed-Monomer
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-TetO-Wt1

    Plasmid

    #41082

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Wt1 (-KTS alternative isoform) (Wt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO + minimal CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-cdc2-AF (718)

    Plasmid

    #39872

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    Cdc2 (CDK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AF = T14A, Y15F

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

18. T. aq RNA Polymerase

    Plasmid

    #215349

    Purpose

    Express T. aq RNA Polymerase subunits in E. coli cells

    Depositor

    Julius Lucks

    Article

    Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

    Insert

    RNA polymerase
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZ149-pcDNA3-BhCas12b_v4

    Plasmid

    #122446

    Purpose

    Expresses BhCas12b_v4 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BhCas12b_v4-NLS-3xHA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC008 - pBR322 with tetR-inducible RFP

    Plasmid

    #79157

    Purpose

    inducible RFP expression vector

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    TetR + RFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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