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pCNL-C1_RhoB

Plasmid

#65697

Purpose

Expresses CNL-RhoB in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

RhoB (RHOB Human)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only
mCherry-FKBP-SAC1C389S-HLx8

Plasmid

#108138

Purpose

Recruitable inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pPK-351

Plasmid

#157921

Purpose

pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

Depositor

Phillip Kyriakakis

Article

Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

Inserts

PIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD

Use

Synthetic Biology; Optogenetics

Tags

HA tag and SV40NLS

Expression

Mammalian

Mutation

pif3 1-523

Promoter

CMV

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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p6H-SUMO3-ORF24-NTD-Strep

Plasmid

#138467

Purpose

Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

SUMO3 and Strep-Tag II

Expression

Bacterial

Mutation

Amino acids 2-201

Promoter

T7

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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mADAM8 deltaMP in pQCXIP cFLAG

Plasmid

#19149

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM8 (Adam8 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 228-397

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pDECKO-mCherry TFRC_B

Plasmid

#78544

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-GFP

Plasmid

#124667

Purpose

Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-5

Plasmid

#109011

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #5 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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p35S::HY5

Plasmid

#135233

Purpose

constitutive expression of HY5 from A. thaliana (AT5G11260; downregulates UPR signaling)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

HY5 (HY5 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pNB785

Plasmid

#60164

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.2

Plasmid

#78541

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.4

Plasmid

#78543

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pNB787

Plasmid

#60152

Purpose

Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LEU1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pfast bac-flag Fbxl3

Plasmid

#51894

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl3 (Fbxl21 Mouse)

Tags

FLAG

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pLTR PKC epsilon

Plasmid

#8420

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pNB789

Plasmid

#60166

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

RNR1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pMTH PKC gamma

Plasmid

#8412

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Protein Kinase C gamma (Prkcg Rat)

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pLTR PKC gamma

Plasmid

#8418

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Protein Kinase C gamma (Prkcg Rat)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pET-51b(+)_His10x-TEV-Rho-tag

Plasmid

#249665

Purpose

Expression of rhodamine-binding protein tag in bacterial cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

Rho-tag

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pYO1704

Plasmid

#252704

Purpose

Protein expression of GST-HsLC3B (1-120) in bacteria

Depositor

Roger Williams

Article

van Vliet et al J Cell Biol. 2026 Mar 2;225(3):e20250708

Insert

GST-HsLC3B (1-120) (MAP1LC3B Human)

Expression

Bacterial

Mutation

1-120

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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