166,034 results

1. SP1-SB100X

   Plasmid

   #154887

   Purpose

   Sleeping Beauty transposase fused to SP1

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   SP1-Sleeping Beauty
   

   Expression

   Mammalian

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-mScarlet-EFS-Puro

   Plasmid

   #227259

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA63-GG

   Plasmid

   #208015

   Purpose

   for cloning homology arms, with GFP as counter selection marker

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Insert

   msfGFP
   

   Promoter

   14G

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-RIAM

   Plasmid

   #80030

   Purpose

   Mammalian expression of mCherry-RIAM (FL)

   Depositor

   Chinten James Lim

   Insert

   RIAM (APBB1IP Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pSimpleII-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47867

   Purpose

   This plasmid encode NmCas9 with two NLS and a HA tag

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLIX402 PHF2

   Plasmid

   #91700

   Purpose

   inducible lentiviral expression of PHF2

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   PHF2 (PHF2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLHCX-XBP1 mNeonGreen NLS

   Plasmid

   #115971

   Purpose

   ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   X-box binding protein 1 (XBP1 Synthetic, Human)
   

   Use

   Retroviral

   Tags

   HA tag, c-myc NLS, and mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Parkin-IRES-zeo

   Plasmid

   #61727

   Purpose

   Expression of human Parkin fused to mCherry

   Depositor

   Douglas Green, Stephen Tait

   Article

   Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

   Insert

   Parkin (PRKN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 GST TEV LIC cloning vector (2G-T)

   Plasmid

   #29707

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, GST, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104911

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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