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pLIB-TetR-KRAB-IRES-BLAS

Plasmid

#55820

Purpose

retroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Insert

TetR-KRAB

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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betaRBPIG

Plasmid

#16422

Depositor

Juergen Hescheler

Article

Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

Insert

loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pKM808

Plasmid

#134181

Purpose

Lentiviral sgRNA plasmid with blasticidin resistance

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Blast

Plasmid

#175270

Purpose

MegaGate destination with C-terminal HA tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112924

Purpose

piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Piggybac transposon vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPy-CAG-Cre::ERT2-IRES-BSD

Plasmid

#48760

Purpose

CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Cre::ERT2

Use

Ppy

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Blast

Plasmid

#175271

Purpose

MegaGate destination with C-terminal DD tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

Destabilization Domain

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTS2402-Tier1-PhCMV-BlastR

Plasmid

#169494

Purpose

Tier-1 vector encoding PhCMV-driven blasticidin resistance gene BlastR (PhCMV-BlastR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven blasticidin-S deaminase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-2-s2193

Plasmid

#166750

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Human ferritin

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pDual_dsCas9_Blast_hs_NC_chr1

Plasmid

#214690

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

Depositor

Kevin Janes

Insert

dgRNA_Chr1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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