We narrowed to 17,390 results for: bon

1. EF1a-dCas9-KRAB-GFP backbone

   Plasmid

   #194281

   Purpose

   Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. P2A-NLS-tdtomato-EPG backbone

   Plasmid

   #215548

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. RNA motif plasmid cloning backbone

   Plasmid

   #107253

   Purpose

   RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   RNA motif plasmid cloning backbone
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCLc-MND-A0201-SABR-Backbone

   Plasmid

   #119050

   Purpose

   3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

   Depositor

   David Baltimore

   Article

   Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MND

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

   Plasmid

   #160736

   Purpose

   Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCLc-MND-B2705-SABR-Backbone

   Plasmid

   #119051

   Purpose

   3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

   Depositor

   David Baltimore

   Article

   Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. library backbone 3'LTR gRNA and barcode

   Plasmid

   #127397

   Purpose

   Digestion by BstXI and BamHI to release the backbone

   Depositor

   Xiaowei Zhuang

   Article

   Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Puro-mCherry

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Donor-attp-u6-blank-sgrna-backbone

   Plasmid

   #234076

   Purpose

   Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Insert

   sgRNA-Blank
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXTK-BB3 [Backbone_repABC-pMlb-KanR_PartTypeBB]

   Plasmid

   #229389

   Purpose

   XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR_PartTypeBB and an sfGFP dropout

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXTK-BB2 [Backbone_RK2-KanR-TetR_PartTypeBB]

    Plasmid

    #229388

    Purpose

    XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR-TetR_PartTypeBB and an sfGFP dropout

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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