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pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

Plasmid

#160622

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s_MS2:EDLL_Tnos

Use

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-800)-Strep

Plasmid

#141344

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1-His6-TEV-ALIX(703-800)-Strep

Use

Tags

GB1-His6-TEV and Strep-tag (WSHPQFEK)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-RtoK

Plasmid

#194559

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > K" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R > K substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-KtoR

Plasmid

#194560

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "K > R" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K > R substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [M1_n-1] (GB1210)

Plasmid

#75411

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [M1_n-1]

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(800-868)-strep

Plasmid

#180023

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)

Use

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [D1_n-1] (GB1208)

Plasmid

#75409

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [D1_n-1]

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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HisGB1-Hif1a(776-826)_FIH

Plasmid

#99508

Purpose

Bacterial expression of hydroxylated Hif1a TAD (776-826)

Depositor

Peter Wright

Article

Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

Inserts

Hif1a (776-826) (HIF1A Human)
FIH1 (HIF1AN Human)

Use

Tags

His6GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pUPD2 nickase D10A Cas9 (GB1690)

Plasmid

#160587

Purpose

Nickase D10A Cas9 for Nt and Ct fusion.

Depositor

Diego Orzaez

Insert

nickase D10A Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [2_n-1] (GB1206)

Plasmid

#75407

Purpose

tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [2_n-1]

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [D1_2] (GB1205)

Plasmid

#75406

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-ALFANb-HaloTag-GB1-dWPRE

Plasmid

#231600

Purpose

Express ALFANb-HaloTag

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

ALFANb-HaloTag

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) C90A, T172C

Plasmid

#217983

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

C90A, T172C

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) A50C,C90A

Plasmid

#217985

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

A50C, C90A

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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GB1-His-TEV-A7-Strep (2-180)

Plasmid

#190067

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Use

Tags

GB1-His-TEV and Strep

Expression

Bacterial

Mutation

PRD only, residues 2-180

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G175R)-Strep

Plasmid

#196211

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G175R

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G189E)-Strep

Plasmid

#196212

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G189E

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-CEP55(160-217, S215C)

Plasmid

#186793

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CEP55(160-217, S215C)

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-CEP55(160-217, S215C) (CEP55 Human)

Use

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

S215C

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G)

Plasmid

#180029

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)

Use

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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H6GB1tev-IntC-P53(304-393)

Plasmid

#200315

Purpose

bacterial expression of P53 TETCTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Use

Tags

His6GB1tev and designed intein C

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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