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We narrowed to 1,318 results for: V2

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Showing: 201 - 220 of 1318 results

  1. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid
    #225755
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Publication
    Walton et al bioRxiv [Preprint]. 2024 Mar 17:2024.03.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225755-plasmid-map-sequence-id-446065

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  2. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    100903-plasmid-map-sequence-id-196607

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  4. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Igor Ulitsky
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    141104-plasmid-map-sequence-id-298612

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  10. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Publication
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  17. pGMC00009 (aka pRC0494)

    Plasmid
    #195294
    Purpose
    Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195294-plasmid-map-sequence-id-385588

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  18. pαH-S-GSAS-B.1.351.v2

    Plasmid
    #173786
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 2
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    173786-plasmid-map-sequence-id-340773

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  19. pGMC00010 (aka pRC0495)

    Plasmid
    #195295
    Purpose
    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195295-plasmid-map-sequence-id-387224

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Showing: 201 - 220 of 1318 results